Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
N-Myc CRISPR/Cas9 KO Plasmid (m): sc-421918
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- N-Myc CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the N-Myc genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: N-Myc Antibody (B8.4.B): sc-53993
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
N-Myc CRISPR/Cas9 KO Plasmid (m) | sc-421918 | 20 µg | $397.00 |
Overview
Mycn encodes the N-Myc transcription factor, a member of the MYC family that binds E-box motifs to regulate gene programs controlling cell-cycle progression, biosynthetic metabolism, ribosome biogenesis, and lineage determination during embryonic development. In mouse systems, N-Myc is critical for neural and mesenchymal progenitor expansion and coordinates signaling inputs from pathways such as PI3K–AKT–mTOR and MAPK to modulate proliferation and differentiation. Dysregulated MYCN activity is strongly linked to oncogenic transformation, altered chromatin state, and replication stress, making it a central node in studies of tumorigenesis and developmental biology. N-Myc-dependent networks also intersect with DNA damage responses and apoptosis, enabling mechanistic interrogation of growth control and cell fate decisions.
N-Myc CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mycn gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mycn together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mycn open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish N-Myc protein expression.
This CRISPR knockout system enables efficient generation of Mycn-deficient cell models for investigation of N-Myc signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mycn exon(s) critical for N-Myc function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mycn genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by N-Myc CRISPR/Cas9 KO Plasmid (m) and N-Myc CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mycn locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by N-Myc HDR Plasmid (m) and N-Myc HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mycn homology arms to support homology-directed repair at defined Mycn target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.