Date published: 2025-9-5
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N-Myc Antibody (B8.4.B) - Western Blotting - Image 374255
N-Myc 抗体 (B8. 4. B): sc-53993. IMR-32 全细胞裂解液中 N-Myc 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
N-Myc 抗体 (B8. 4. B): sc-53993. IMR-32 全细胞裂解液中 N-Myc 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

N-Myc 抗体 (B8.4.B): sc-53993

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说明书
  • N-Myc 抗体 B8.4.B 是小鼠单克隆 IgG2a κ，N-Myc抗体, 在209篇文献中引用，规格为200 µg/ml
  • 针对N-terminal重组N-Myc的human来源
  • N-Myc 抗体 (B8.4.B) 推荐用于 WB 和 IP，检测mouse, rat 和human 来源的 N-Myc
  • 抗N-Myc抗体（B8.4.B）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取N-Myc (B8.4.B): sc-53993的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是N-Myc Antibody (B8.4.B) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与N-Myc Antibody (B8.4.B) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Myc抗体，包括c-Myc和N-Myc

N-Myc 抗体（B8.4.B）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过免疫印迹（WB）和免疫沉淀（IP）检测小鼠、大鼠和人源的 N-Myc 蛋白。抗 N-Myc 抗体（B8.4.B）有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 共轭物，可满足不同的实验需求。N-Myc 蛋白是 Myc 转录因子家族的成员，在细胞增殖、分化和凋亡过程中起着至关重要的作用，因此 N-Myc 在肿瘤发生过程中扮演着重要角色。N-Myc失调，特别是通过基因扩增，通常与侵袭性神经母细胞瘤有关，其中N-Myc的表达水平与疾病进展和患者预后相关。N-Myc 蛋白主要位于细胞核中，在细胞核中 N-Myc 发挥转录调节因子的作用，与特定的 DNA 序列结合，调节参与细胞周期控制和生长的靶基因的表达。了解 N-Myc 的定位和功能对于开发旨在抑制癌细胞中 N-Myc 活性的靶向疗法至关重要，从而为治疗 N-Myc 驱动的恶性肿瘤提供了潜在的途径。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

N-Myc 抗体 (B8.4.B) 参考文献:

  1. MAZ是一种锌指蛋白，能与c-MYC和C2基因序列结合，调节转录的启动和终止。  |  Bossone, SA., et al. 1992. Proc Natl Acad Sci U S A. 89: 7452-6. PMID: 1502157
  2. Src家族激酶和N-Myc在精原干细胞增殖中的作用  |  Braydich-Stolle, L., et al. 2007. Dev Biol. 304: 34-45. PMID: 17222400
  3. N-MYC 调节人神经母细胞瘤中局灶粘附激酶的表达。  |  Beierle, EA., et al. 2007. J Biol Chem. 282: 12503-16. PMID: 17327229
  4. Myn（max 的小鼠同源物）与 c-Myc 的结合可刺激甲基化敏感 DNA 结合和 ras 共转化。  |  Prendergast, GC., et al. 1991. Cell. 65: 395-407. PMID: 1840505
  5. Max：一种螺旋-环-螺旋拉链蛋白，与 Myc 形成序列特异的 DNA 结合复合物。  |  Blackwood, EM. and Eisenman, RN. 1991. Science. 251: 1211-7. PMID: 2006410
  6. myc 致癌基因：在转化和分化中的作用。  |  Cole, MD. 1986. Annu Rev Genet. 20: 361-84. PMID: 3028245
  7. N-Myc诱导的代谢重置为神经母细胞瘤创造了新的治疗弱点。  |  Tjaden, B., et al. 2020. Sci Rep. 10: 7157. PMID: 32346009
  8. N-Myc通过调控FSCN1诱导前列腺癌的肿瘤进展。  |  He, G., et al. 2020. Oncol Rep. 44: 2265-2274. PMID: 33000273
  9. PHA-680626 是 Aurora-A 与 N-Myc 相互作用的有效抑制剂  |  Boi, D., et al. 2021. Int J Mol Sci. 22: PMID: 34884931
  10. N-Myc与STAT互作蛋白是子宫内膜异位症的抑制因子。  |  Park, Y., et al. 2024. Int J Mol Sci. 25: PMID: 39125716
  11. 未经治疗的人类神经母细胞瘤中的 N-myc 扩增与疾病晚期有关。  |  Brodeur, GM., et al. 1984. Science. 224: 1121-4. PMID: 6719137
  12. 人类神经母细胞瘤细胞系和一种神经母细胞瘤肿瘤共享与 myc 细胞癌基因同源性有限的扩增 DNA。  |  Schwab, M., et al. Nature. 305: 245-8. PMID: 6888561

订购信息

产品名称产品编号规格价格数量收藏夹

N-Myc 抗体 (B8.4.B)

sc-53993
200 µg/ml
$316.00

N-Myc (B8.4.B): m-IgG Fc BP-HRP 套装

sc-528591
200 µg Ab; 10 µg BP
$354.00

N-Myc (B8.4.B): m-IgGκ BP-HRP 套装

sc-521004
200 µg Ab, 40 µg BP
$354.00

N-Myc (B8.4.B): m-IgG2a BP-HRP 套装

sc-547126
200 µg Ab; 10 µg BP
$354.00

N-Myc 抗体 (B8.4.B) AC

sc-53993 AC
500 µg/ml, 25% agarose
$416.00

N-Myc 抗体 (B8.4.B) HRP

sc-53993 HRP
200 µg/ml
$316.00

N-Myc 抗体 (B8.4.B) FITC

sc-53993 FITC
200 µg/ml
$330.00

N-Myc 抗体 (B8.4.B) PE

sc-53993 PE
200 µg/ml
$343.00

N-Myc 抗体 (B8.4.B) Alexa Fluor® 488

sc-53993 AF488
200 µg/ml
$357.00

N-Myc 抗体 (B8.4.B) Alexa Fluor® 546

sc-53993 AF546
200 µg/ml
$357.00

N-Myc 抗体 (B8.4.B) Alexa Fluor® 594

sc-53993 AF594
200 µg/ml
$357.00

N-Myc 抗体 (B8.4.B) Alexa Fluor® 647

sc-53993 AF647
200 µg/ml
$357.00

N-Myc 抗体 (B8.4.B) Alexa Fluor® 680

sc-53993 AF680
200 µg/ml
$357.00

N-Myc 抗体 (B8.4.B) Alexa Fluor® 790

sc-53993 AF790
200 µg/ml
$357.00

Hi, Can you share more information on the epitope used to generate this antibody? If the detailed epitope information is confidential, please share the general region where the epitope is located Thanks

Asked by: CCYuan
Thank you for your question. sc-53993 N-Myc (B8.4.B) has not been epitope mapped. Here is a link to more information on the protein. https://www.uniprot.org/uniprotkb/P04198/entry
Answered by: BlakeJ
Date published: 2024-08-14

can I use this antibody for ICC

Asked by: Jeew
Thank you for your question. The conjugated forms of this antibody, sc-53993, is warrantied for IF and IHC applications, but the unconjugated form is only warrantied for WB and IP use. We do have publications for IF/IHC, with this antibody, you can find them here: https://www.scbt.com/scbt/misc/citationResults.jsp?_requestid=476978 If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-03-22

What is the recommended concentration for use in IHC?

Asked by: Steev
Thank you for your question. We recommend using this mouse monoclonal antibody, N-Myc (B8.4.B): sc-53993, at a starting dilution of 1:50 (range of 1:50-1:500) for immunohistochemistry. A link to our recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-10-31

Does this cross react with c-myc?

Asked by: alexbk
Thank you for your question. No, this mouse monoclonal antibody, N-Myc (B8.4.B): sc-53993, is specific for N-Myc and does not cross-react with c-Myc.
Answered by: Technical Support
Date published: 2017-06-01

Would like to know the exact position of the recombinant protein used to raise this antibody. We need this info to interpret data on Cas9/Crisper gene depletion. Thanks

Asked by: Bisu
Thank you for your question. Unfortunately, the sequence of the recombinant protein used to generate this monoclonal antibody, N-Myc (B8.4.B): sc-53993, is not known. The only information describing the immunogen is that it was derived from the N-terminus of N-Myc of human origin. As an alternative, we do offer different mouse monoclonal antibodies against N-Myc where more detailed information is provided about the immunogen(s) used to develop these antibodies. For example, N-Myc (2): sc-142 with epitope mapping at residues 327-339 of N-Myc of human origin. Or N-Myc (C-4): sc-515099 specific for an epitope mapping between amino acids 443-464 within the C-terminus of N-Myc of human origin.
Answered by: Technical Support
Date published: 2017-01-16

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: AbPolly
Thank you for your inquiry. Instead of a standard anti-mouse secondary antibody, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2016-12-20

Are there any issues in using this mouse monoclonal antibody to stain mouse tissues in IF or IHC?

Asked by: AbPolly
Thank you for your inquiry. This antibody can be used for staining mouse tissues. We recommend using a directly conjugated mouse monoclonal primary antibody. This N-Myc mouse monoclonal antibody is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Service
Date published: 2016-12-20
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Rated 5 out of 5 by from Works great for ChIP-seqAntibody works great for N-Myc ChIP-seq studies with no cross-reactivity against c-Myc.
Date published: 2023-07-05
Rated 5 out of 5 by from good anitbodyMYCN b8.4.b recognizes a single band in D.rerio lysate at the predicted molecular weight.
Date published: 2021-04-20
Rated 5 out of 5 by from This antibody worked well for IFThis antibody worked well for IF, using human neuroblastoma cell lines which have high endogenous N-Myc levels.
Date published: 2019-08-12
Rated 5 out of 5 by from Good antibodyI tried a sample of this antibody and I found it works very well for WB. I made a 1:500 dilution for the detection of N-MYC in the SK-N-BE(2) cell line. I got a very strong signal and I project that I'm going to be able to work with bigger dilutions, such as 1:2000. The band appeared between 55 and 72 kDa, which conforms with the molecular weight of the protein, 67 kDa.
Date published: 2019-01-24
Rated 4 out of 5 by from Clean Bands, Probes Very WellAntibody produces clean, well-defined bands when used to detect N-Myc in human cancer cells, at a 1:1000 dilution.
Date published: 2018-01-04
Rated 2 out of 5 by from Not workNo band detected in 293FT transfected with N-MYC plamid
Date published: 2017-09-15
Rated 5 out of 5 by from Very good antibodyThis N-Myc antibody works very well for chromatin immunoprecipitation.
Date published: 2017-09-13
Rated 4 out of 5 by from not suitable for turbotthere is no band when i exposed for 25min ,dulited for 1:100
Date published: 2017-07-10
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N-Myc 抗体 (B8.4.B) is rated 4.7 out of 5 by 24.
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