Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
N-CoR CRISPR/Cas9 KO Plasmid (m): sc-422771
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- N-CoR CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the N-CoR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: N-CoR Antibody (F-1): sc-515934
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
N-CoR CRISPR/Cas9 KO Plasmid (m) | sc-422771 | 20 µg | $397.00 |
Overview
Mouse Ncor1 encodes nuclear receptor corepressor 1 (N‑CoR), a scaffold protein that assembles transcriptional repression complexes with HDAC3, TBL1/TBLR1, and other factors to regulate chromatin accessibility and gene expression. N‑CoR modulates nuclear receptor signaling and broader transcriptional networks governing differentiation, metabolic homeostasis, and immune cell programming, including regulation of inflammatory gene sets. Through coordinated deacetylation and corepressor recruitment, Ncor1 impacts lineage commitment and cellular responses to hormonal and cytokine cues. Dysregulation of N‑CoR–dependent repression has been linked to aberrant transcriptional states relevant to cancer biology, neurodevelopmental phenotypes, and immune dysfunction in experimental models.
N-CoR CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ncor1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ncor1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ncor1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish N-CoR protein expression.
This CRISPR knockout system enables efficient generation of Ncor1-deficient cell models for investigation of N-CoR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ncor1 exon(s) critical for N-CoR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ncor1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by N-CoR CRISPR/Cas9 KO Plasmid (m) and N-CoR CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ncor1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by N-CoR HDR Plasmid (m) and N-CoR HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ncor1 homology arms to support homology-directed repair at defined Ncor1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.