N-cadherin Antibody (H-2)recommended for detection of N-cadherin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

N-cadherin Antibody (H-2): sc-393933

N-cadherin Antibody (H-2) is rated 4.9 out of 5 by 9.
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Datasheets

    • N-cadherin Antibody (H-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 800-825 within a C-terminal cytoplasmic domain of N-cadherin of human origin
    • N-cadherin Antibody (H-2) is recommended for detection of N-cadherin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • N-cadherin Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-393933 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of N-cadherin (H-2): sc-393933.
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想问一下怎么申请试用装 sc-393933 AF488 和 N-cadherin Antibody (H-2)

Asked by: pzfei
感谢您的提问。关于试用装的申请,欢迎通过:电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For sample request, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-04-23

Can you recommend a positive control to use with N-cadherin (H-2): sc-393933 monoclonal antibody for Western blot?

Asked by: jenni
We recommend mouse brain (sc-2253) and human heart (sc-363763) tissue extracts to use as positive controls as we have great Western blot data showing specific detection of Calnexin in these cell lines.
Answered by: Technical Support 2
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with N-cadherin (H-2): sc-393933?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-12
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Rated 5 out of 5 by from Great for both IF and WB I tested out the sample version of this antibody for both WB (1:500) and IF (1:250) and got a strong signal. The cell junction localization looks great by both 10x and 20x imaging.
Date published: 2019-02-08
Rated 5 out of 5 by from Good signal (inmunofluorescence) We have used for IF and we obtained a good fluorescence signal (1:50 dilution)
Date published: 2018-03-07
Rated 5 out of 5 by from Great features It's great for my WB. N-cad had changed with my treatment for C4-2 cell line.
Date published: 2017-11-12
Rated 5 out of 5 by from Good job We got just a sample to test and it worked so great that now we bought it! It works in WB and IF.
Date published: 2017-11-07
Rated 4 out of 5 by from Nice IF I tested on immunofluorescence and it worked well. nice membrane staining.
Date published: 2017-11-01
Rated 5 out of 5 by from Works well It works well and worth recommending~~~~~~~~~~~~~~~~~~~~~~~~~~
Date published: 2017-06-21
Rated 5 out of 5 by from Superb western and IHC Strong clear western blot results; also a great product for IHC staining
Date published: 2017-01-30
Rated 5 out of 5 by from Great N-cadherin antibody This N-cadherin antibody produces strong signal and is cross-reactive with mouse, rat, and human samples. Good option for N-cadherin detection by WB, IP, IF, IHC(P), or ELISA.
Date published: 2017-01-24
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