Myotrophin Antibody (D-3): sc-166072

Myotrophin Antibody (D-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects Myotrophin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Myotrophin (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myotrophin, also known as V-1 protein, is a cytoplasmic protein that plays a crucial role in cellular signaling and is involved in the differentiation of cerebellar neurons, particularly granule cells. This 117 amino acid protein is characterized by its acetylated N-terminus and contains two internal ankyrin repeats, which are important for protein-protein interactions and cellular localization. Myotrophin is notably upregulated in response to mechanical stress in myocytes, such as cyclic stretch, and during inflammatory responses mediated by cytokines like tumor necrosis factor α (TNFα) and interleukin-1β. Expression is highest in the brain, indicating a significant role in neural development and function, while lower levels are found in skeletal muscle. Notably, Myotrophin has been associated with cardiac hypertrophy, as Myotrophin can induce the expression of protooncogenes and cardiac-specific genes, making this protein a key player in both normal physiological processes and pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Myotrophin Antibody (D-3) References:

1. cDNA sequence and characterization of the gene that encodes human myotrophin/V-1 protein, a mediator of cardiac hypertrophy.  |  Anderson, KM., et al. 1999. J Mol Cell Cardiol. 31: 705-19. PMID: 10329199
2. Myotrophin/V-1, a protein up-regulated in the failing human heart and in postnatal cerebellum, converts NFkappa B p50-p65 heterodimers to p50-p50 and p65-p65 homodimers.  |  Knuefermann, P., et al. 2002. J Biol Chem. 277: 23888-97. PMID: 11971907
3. Myotrophin-kappaB DNA interaction in the initiation process of cardiac hypertrophy.  |  Gupta, S. and Sen, S. 2002. Biochim Biophys Acta. 1589: 247-60. PMID: 12031792
4. Glucocorticoid inhibits expression of V-1, a catecholamine biosynthesis regulatory protein, in cultured adrenal medullary cells.  |  Hiwatashi, Y., et al. 2002. FEBS Lett. 528: 166-70. PMID: 12297298
5. Myocardial cell death and regeneration during progression of cardiac hypertrophy to heart failure.  |  Sarkar, S., et al. 2004. J Biol Chem. 279: 52630-42. PMID: 15385543
6. Regulation of myotrophin gene by pressure overload and stretch.  |  Sil, P., et al. 2004. Mol Cell Biochem. 262: 79-89. PMID: 15532712
7. A pancreatic islet-specific microRNA regulates insulin secretion.  |  Poy, MN., et al. 2004. Nature. 432: 226-30. PMID: 15538371
8. Centrally administered vasopressin antagonizes pentobarbital-induced narcosis and depression of hippocampal cholinergic activity.  |  Horita, A. and Carino, MA. 1990. Peptides. 11: 1021-5. PMID: 1980941
9. Myotrophin: purification of a novel peptide from spontaneously hypertensive rat heart that influences myocardial growth.  |  Sen, S., et al. 1990. J Biol Chem. 265: 16635-43. PMID: 2144530
10. Myotrophin induces early response genes and enhances cardiac gene expression.  |  Mukherjee, DP., et al. 1993. Hypertension. 21: 142-8. PMID: 8428777
11. Myotrophin in human cardiomyopathic heart.  |  Sil, P., et al. 1993. Circ Res. 73: 98-108. PMID: 8508536
12. Cardiac myotrophin exhibits rel/NF-kappa B interacting activity in vitro.  |  Sivasubramanian, N., et al. 1996. J Biol Chem. 271: 2812-6. PMID: 8576259

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myotrophin Antibody (D-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Myotrophin Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Myotrophin Antibody (D-3) Specifications

Alternate Product Name: Myotrophin

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 74-110

Epitope Target: Myotrophin

Epitope Species: human

Product Reactivity/Detection: Myotrophin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Myotrophin Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin