Myosin X Antibody (C-1): sc-166720

Myosin X Antibody (C-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Myosin X protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Myosin X antibody (C-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myosin X, also known as Myo10 or M10, plays a crucial role in cellular motility and is involved in processes such as phagocytosis, where Myosin X facilitates the movement of cellular components along filamentous actin. Myosin X is characterized by a unique structure, which includes three IQ motifs, a Myosin tail homology 4 (MyTH4) domain, a FERM (band 4.1/Ezrin/Radixin/Moesin) domain, three Pleckstrin homology domains that mediate phosphatidylinositol phospholipid signaling, and three PEST sites that may allow for the cleavage of the Myosin tail. This structural complexity enables Myosin X to bind to F-actin in an ATP-sensitive manner, thereby influencing the dynamics of actin filaments during cellular processes. In cultured cells, Myosin X localizes to the edges of lamellipodia, membrane ruffles, and the tips of filopodial actin bundles, highlighting Myosin X′s importance in cellular architecture and movement. The human Myosin X gene is located on chromosome 5p15.1-p14.3 and encodes a protein consisting of 2,058 amino acids, underscoring Myosin X′s significance in various physiological and pathological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

Download thiomersal SDS

Myosin X Antibody (C-1) References:

1. Myosin-X, a novel myosin with pleckstrin homology domains, associates with regions of dynamic actin.  |  Berg, JS., et al. 2000. J Cell Sci. 113 Pt 19: 3439-51. PMID: 10984435
2. Motor function and regulation of myosin X.  |  Homma, K., et al. 2001. J Biol Chem. 276: 34348-54. PMID: 11457842
3. Myosin-X is an unconventional myosin that undergoes intrafilopodial motility.  |  Berg, JS. and Cheney, RE. 2002. Nat Cell Biol. 4: 246-50. PMID: 11854753
4. Myosin X is a downstream effector of PI(3)K during phagocytosis.  |  Cox, D., et al. 2002. Nat Cell Biol. 4: 469-77. PMID: 12055636
5. May the force be with you: Myosin-X in phagocytosis.  |  Chavrier, P. 2002. Nat Cell Biol. 4: E169-71. PMID: 12105426
6. The myosin X motor is optimized for movement on actin bundles.  |  Ropars, V., et al. 2016. Nat Commun. 7: 12456. PMID: 27580874
7. The Antiparallel Dimerization of Myosin X Imparts Bundle Selectivity for Processive Motility.  |  Caporizzo, MA., et al. 2018. Biophys J. 114: 1400-1410. PMID: 29590597
8. Myosin X Interaction with KIF13B, a Crucial Pathway for Netrin-1-Induced Axonal Development.  |  Yu, HL., et al. 2020. J Neurosci. 40: 9169-9185. PMID: 33097641
9. Myosin-X is dispensable for spindle morphogenesis and positioning in the mouse oocyte.  |  Crozet, F., et al. 2021. Development. 148: PMID: 33722900
10. Increased expression of Myosin X contributes to the metastasis in patients with laryngeal squamous cell carcinoma.  |  Deng, G., et al. 2022. Mol Genet Genomics. 297: 1529-1536. PMID: 35951144
11. The Antiparallel Coiled-Coil Domain Allows Multiple Forward Step Sizes of Myosin X.  |  Nguyen, QQ., et al. 2023. J Phys Chem Lett. 14: 4914-4922. PMID: 37202741
12. Mapping of unconventional myosins in mouse and human.  |  Hasson, T., et al. 1996. Genomics. 36: 431-9. PMID: 8884266

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myosin X Antibody (C-1) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Myosin X Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Myosin X Antibody (C-1) Specifications

Alternate Product Name: Myosin X

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1759-2058

Epitope Target: Myosin X

Epitope Species: human

Product Reactivity/Detection: Myosin X

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Myosin X Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin