Myosin X Antibody (C-1): sc-166720
- Myosin X Antibody (C-1) is a mouse monoclonal IgG2b κ Myosin X antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 1759-2058 mapping at the C-terminus of Myosin X of human origin
- Myosin X Antibody (C-1) is recommended for detection of Myosin X of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Myosin X Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Myosin X Antibody (C-1) for WB and IHC(P) applications. These reagents are now offered in bundles with Myosin X Antibody (C-1) (see ordering information below).
Myosin X Antibody (C-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Myosin X protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Myosin X antibody (C-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myosin X, also known as Myo10 or M10, plays a crucial role in cellular motility and is involved in processes such as phagocytosis, where Myosin X facilitates the movement of cellular components along filamentous actin. Myosin X is characterized by a unique structure, which includes three IQ motifs, a Myosin tail homology 4 (MyTH4) domain, a FERM (band 4.1/Ezrin/Radixin/Moesin) domain, three Pleckstrin homology domains that mediate phosphatidylinositol phospholipid signaling, and three PEST sites that may allow for the cleavage of the Myosin tail. This structural complexity enables Myosin X to bind to F-actin in an ATP-sensitive manner, thereby influencing the dynamics of actin filaments during cellular processes. In cultured cells, Myosin X localizes to the edges of lamellipodia, membrane ruffles, and the tips of filopodial actin bundles, highlighting Myosin X′s importance in cellular architecture and movement. The human Myosin X gene is located on chromosome 5p15.1-p14.3 and encodes a protein consisting of 2,058 amino acids, underscoring Myosin X′s significance in various physiological and pathological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Myosin X Antibody (C-1) References:
- Myosin-X, a novel myosin with pleckstrin homology domains, associates with regions of dynamic actin. | Berg, JS., et al. 2000. J Cell Sci. 113 Pt 19: 3439-51. PMID: 10984435
- Motor function and regulation of myosin X. | Homma, K., et al. 2001. J Biol Chem. 276: 34348-54. PMID: 11457842
- Myosin-X is an unconventional myosin that undergoes intrafilopodial motility. | Berg, JS. and Cheney, RE. 2002. Nat Cell Biol. 4: 246-50. PMID: 11854753
- Myosin X is a downstream effector of PI(3)K during phagocytosis. | Cox, D., et al. 2002. Nat Cell Biol. 4: 469-77. PMID: 12055636
- May the force be with you: Myosin-X in phagocytosis. | Chavrier, P. 2002. Nat Cell Biol. 4: E169-71. PMID: 12105426
- The myosin X motor is optimized for movement on actin bundles. | Ropars, V., et al. 2016. Nat Commun. 7: 12456. PMID: 27580874
- The Antiparallel Dimerization of Myosin X Imparts Bundle Selectivity for Processive Motility. | Caporizzo, MA., et al. 2018. Biophys J. 114: 1400-1410. PMID: 29590597
- Myosin X Interaction with KIF13B, a Crucial Pathway for Netrin-1-Induced Axonal Development. | Yu, HL., et al. 2020. J Neurosci. 40: 9169-9185. PMID: 33097641
- Myosin-X is dispensable for spindle morphogenesis and positioning in the mouse oocyte. | Crozet, F., et al. 2021. Development. 148: PMID: 33722900
- Increased expression of Myosin X contributes to the metastasis in patients with laryngeal squamous cell carcinoma. | Deng, G., et al. 2022. Mol Genet Genomics. 297: 1529-1536. PMID: 35951144
- The Antiparallel Coiled-Coil Domain Allows Multiple Forward Step Sizes of Myosin X. | Nguyen, QQ., et al. 2023. J Phys Chem Lett. 14: 4914-4922. PMID: 37202741
- Mapping of unconventional myosins in mouse and human. | Hasson, T., et al. 1996. Genomics. 36: 431-9. PMID: 8884266
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myosin X Antibody (C-1) | sc-166720 | 200 µg/ml | $322.00 | |||
Myosin X Antibody (C-1): m-IgGκ BP-HRP Bundle | sc-521722 | 200 µg Ab, 40 µg BP | $361.00 | |||
Myosin X Antibody (C-1): m-IgG2b BP-HRP Bundle | sc-548960 | 200 µg Ab; 10 µg BP | $361.00 | |||
Myosin X Antibody (C-1) AC | sc-166720 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Myosin X Antibody (C-1) HRP | sc-166720 HRP | 200 µg/ml | $322.00 | |||
Myosin X Antibody (C-1) FITC | sc-166720 FITC | 200 µg/ml | $336.00 | |||
Myosin X Antibody (C-1) PE | sc-166720 PE | 200 µg/ml | $349.00 | |||
Myosin X Antibody (C-1) Alexa Fluor® 488 | sc-166720 AF488 | 200 µg/ml | $364.00 | |||
Myosin X Antibody (C-1) Alexa Fluor® 546 | sc-166720 AF546 | 200 µg/ml | $364.00 | |||
Myosin X Antibody (C-1) Alexa Fluor® 594 | sc-166720 AF594 | 200 µg/ml | $364.00 | |||
Myosin X Antibody (C-1) Alexa Fluor® 647 | sc-166720 AF647 | 200 µg/ml | $364.00 | |||
Myosin X Antibody (C-1) Alexa Fluor® 680 | sc-166720 AF680 | 200 µg/ml | $364.00 | |||
Myosin X Antibody (C-1) Alexa Fluor® 790 | sc-166720 AF790 | 200 µg/ml | $364.00 |