Myosin Va Antibody (G-4): sc-365986

Myosin Va Antibody (G-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Myosin Va (MYO5A) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-Myosin Va antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myosin Va (G-4) monoclonal antibody recognizes a member of the unconventional myosin family that plays a crucial role in intracellular transport, particularly in moving organelles and vesicles along actin filaments. This transport maintains cellular function and organization by facilitating delivery of essential materials to specific locations within cells, influencing processes such as cell signaling, growth, and division. Myosin Va′s unique structure combines features of both nonmuscle myosin type I and type II, enabling effective interaction with actin filaments for motor activity. Phosphorylation of Myosin Va′s heavy chain in the carboxy-terminal tail domain regulates activity and protein interactions. Mutations in Myosin Va genes cause coat color dilution in mice, severe neurological defects shortly after birth, and Griscelli disease in humans - a rare disorder characterized by pigmentary dilution and immunodeficiency.

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Myosin Va Antibody (G-4) References:

1. High affinity binding of brain myosin-Va to F-actin induced by calcium in the presence of ATP.  |  Tauhata, SB., et al. 2001. J Biol Chem. 276: 39812-8. PMID: 11517216
2. Regulation of nonmuscle myosins by heavy chain phosphorylation.  |  Redowicz, MJ. 2001. J Muscle Res Cell Motil. 22: 163-73. PMID: 11519739
3. Myosin-Va and dynamic actin oppose microtubules to drive long-range organelle transport.  |  Evans, RD., et al. 2014. Curr Biol. 24: 1743-50. PMID: 25065759
4. Myosin Va plays essential roles in maintaining normal mitosis, enhancing tumor cell motility and viability.  |  Li, YR., et al. 2017. Oncotarget. 8: 54654-54671. PMID: 28903372
5. Myosin-Va is required for preciliary vesicle transportation to the mother centriole during ciliogenesis.  |  Wu, CT., et al. 2018. Nat Cell Biol. 20: 175-185. PMID: 29335527
6. LRRK2-phosphorylated Rab10 sequesters Myosin Va with RILPL2 during ciliogenesis blockade.  |  Dhekne, HS., et al. 2021. Life Sci Alliance. 4: PMID: 33727250
7. The kinesin Kif21b binds myosin Va and mediates changes in actin dynamics underlying homeostatic synaptic downscaling.  |  Gromova, KV., et al. 2023. Cell Rep. 42: 112743. PMID: 37418322
8. Myosin Va-dependent Transport of NMDA Receptors in Hippocampal Neurons.  |  Gong, R., et al. 2024. Neurosci Bull. 40: 1053-1075. PMID: 38291290
9. Rab27a GTPase and its effector Myosin Va are host factors required for efficient Oropouche virus cell egress.  |  Concha, JO., et al. 2024. PLoS Pathog. 20: e1012504. PMID: 39213446
10. Cloning, analysis, and chromosomal localization of myoxin (MYH12), the human homologue to the mouse dilute gene.  |  Engle, LJ. and Kennett, RH. 1994. Genomics. 19: 407-16. PMID: 8188282
11. Griscelli disease maps to chromosome 15q21 and is associated with mutations in the myosin-Va gene.  |  Pastural, E., et al. 1997. Nat Genet. 16: 289-92. PMID: 9207796
12. Myosin Va associates with microtubule-rich domains in both interphase and dividing cells.  |  Wu, X., et al. 1998. Cell Motil Cytoskeleton. 40: 286-303. PMID: 9678671

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myosin Va Antibody (G-4) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Myosin Va Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Myosin Va Antibody (G-4) Specifications

Alternate Product Name: Myosin Va

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 40-59

Epitope Target: Myosin Va

Epitope Species: human

Product Reactivity/Detection: Myosin Va

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Myosin Va Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin