myomesin-2 Antibody (E-5): sc-515638
- myomesin-2 Antibody (E-5) is a mouse monoclonal IgG1 κ myomesin-2 antibody provided at 200 µg/ml
- raised against amino acids 913-977 mapping within an internal region of myomesin-2 of human origin
- myomesin-2 Antibody (E-5) is recommended for detection of myomesin-2 of human origin by WB, IP, IF and ELISA
- Anti-myomesin-2 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for myomesin-2 Antibody (E-5) for WB applications. These reagents are now offered in bundles with myomesin-2 Antibody (E-5) (see ordering information below).
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myomesin-2 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects myomesin-2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-myomesin-2 antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. myomesin-2 plays a crucial role in the structural integrity of the sarcomere, specifically within the M band, where myomesin-2 interacts with other key proteins such as Titin, Myosin, and Connectin. This interaction is vital for maintaining the organization and elasticity of muscle fibers, which is essential for proper muscle contraction and overall function. The unique N-terminal domain of myomesin-2, along with expression primarily in skeletal muscle, underscores myomesin-2′s importance in muscle physiology and potential implications in muscle-related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
myomesin-2 Antibody (E-5) References:
- A novel marker for vertebrate embryonic heart, the EH-myomesin isoform. | Agarkova, I., et al. 2000. J Biol Chem. 275: 10256-64. PMID: 10744711
- Postnatal suppression of myomesin, muscle creatine kinase and the M-line in rat extraocular muscle. | Porter, JD., et al. 2003. J Exp Biol. 206: 3101-12. PMID: 12878677
- Muscle-type creatine kinase interacts with central domains of the M-band proteins myomesin and M-protein. | Hornemann, T., et al. 2003. J Mol Biol. 332: 877-87. PMID: 12972258
- A new 185,000-dalton skeletal muscle protein detected by monoclonal antibodies. | Grove, BK., et al. 1984. J Cell Biol. 98: 518-24. PMID: 6537951
- The globular head domain of titin extends into the center of the sarcomeric M band. cDNA cloning, epitope mapping and immunoelectron microscopy of two titin-associated proteins. | Vinkemeier, U., et al. 1993. J Cell Sci. 106 (Pt 1): 319-30. PMID: 7505783
- Assignment of the human gene for the sarcomeric M-band protein myomesin (MYOM1) to 18p11.31-p11.32. | Speel, EJ., et al. 1998. Genomics. 54: 184-6. PMID: 9806852
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
myomesin-2 Antibody (E-5) | sc-515638 | 200 µg/ml | $322.00 | |||
myomesin-2 Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-525142 | 200 µg Ab, 40 µg BP | $361.00 | |||
myomesin-2 Antibody (E-5): m-IgG1 BP-HRP Bundle | sc-533646 | 200 µg Ab; 20 µg BP | $361.00 | |||
myomesin-2 Antibody (E-5) AC | sc-515638 AC | 500 µg/ml, 25% agarose | $424.00 | |||
myomesin-2 Antibody (E-5) HRP | sc-515638 HRP | 200 µg/ml | $322.00 | |||
myomesin-2 Antibody (E-5) FITC | sc-515638 FITC | 200 µg/ml | $336.00 | |||
myomesin-2 Antibody (E-5) PE | sc-515638 PE | 200 µg/ml | $349.00 | |||
myomesin-2 Antibody (E-5) Alexa Fluor® 488 | sc-515638 AF488 | 200 µg/ml | $364.00 | |||
myomesin-2 Antibody (E-5) Alexa Fluor® 546 | sc-515638 AF546 | 200 µg/ml | $364.00 | |||
myomesin-2 Antibody (E-5) Alexa Fluor® 594 | sc-515638 AF594 | 200 µg/ml | $364.00 | |||
myomesin-2 Antibody (E-5) Alexa Fluor® 647 | sc-515638 AF647 | 200 µg/ml | $364.00 | |||
myomesin-2 Antibody (E-5) Alexa Fluor® 680 | sc-515638 AF680 | 200 µg/ml | $364.00 | |||
myomesin-2 Antibody (E-5) Alexa Fluor® 790 | sc-515638 AF790 | 200 µg/ml | $364.00 |