Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
myoglobin CRISPR/Cas9 KO Plasmid (h): sc-401370
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- myoglobin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the myoglobin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: myoglobin Antibody (A-6): sc-393020
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
myoglobin CRISPR/Cas9 KO Plasmid (h) | sc-401370 | 20 µg | $397.00 |
Overview
MB encodes myoglobin, a heme-containing oxygen-binding protein that facilitates intracellular oxygen storage and diffusion in striated muscle, supporting aerobic metabolism during fluctuating oxygen demand. By buffering oxygen availability, myoglobin influences mitochondrial oxidative phosphorylation, redox homeostasis, and responses to hypoxia and reactive nitrogen/oxygen species. Altered MB expression or function has been examined in the context of myopathies, ischemia-related muscle injury, and broader metabolic adaptation of muscle cells. In oncology and cardiovascular biology, myoglobin-associated oxygen handling and oxidative stress pathways are often investigated as determinants of cellular fitness under limited oxygen.
myoglobin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish myoglobin protein expression.
This CRISPR knockout system enables efficient generation of MB-deficient cell models for investigation of myoglobin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MB exon(s) critical for myoglobin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by myoglobin CRISPR/Cas9 KO Plasmid (h) and myoglobin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by myoglobin HDR Plasmid (h) and myoglobin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MB homology arms to support homology-directed repair at defined MB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.