Myocardin Antibody (E-5): sc-518132

Myocardin Antibody (E-5) is a mouse monoclonal IgM antibody that detects Myocardin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Myocardin antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myocardin plays a crucial role in regulating smooth muscle and cardiac muscle gene expression by functioning as a co-activator for serum response factor (SRF), a key transcription factor that binds to serum response elements (SRE) in promoters of many genes responsive to growth signals. The interaction between Myocardin and SRF is particularly important in cardiac muscle cells, where Myocardin activates specific cardiac muscle promoters, thereby influencing heart development and function. Myocardin is expressed in vascular smooth muscle cells, where Myocardin is essential for cell differentiation and maintenance of smooth muscle identity. Myocardin is localized in the nucleus, which is critical for Myocardin′s role in gene regulation, and Myocardin is absent in non-muscle tissues such as coronary vasculature and skeletal muscle, highlighting Myocardin′s specificity in muscle-related functions. Myocardin belongs to the SAP (SAF-A/B, Acinus, and PIAS) domain family of nuclear proteins, which may facilitate targeting of proteins to specific chromosomal locations, further underscoring Myocardin′s significance in cellular processes.

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Myocardin Antibody (E-5) References:

1. SAP - a putative DNA-binding motif involved in chromosomal organization.  |  Aravind, L. and Koonin, EV. 2000. Trends Biochem Sci. 25: 112-4. PMID: 10694879
2. Activation of cardiac gene expression by myocardin, a transcriptional cofactor for serum response factor.  |  Wang, D., et al. 2001. Cell. 105: 851-62. PMID: 11439182
3. The SRE: a growth factor responsive transcriptional regulator.  |  Treisman, R. 1990. Semin Cancer Biol. 1: 47-58. PMID: 2133110
4. The sarcomeric actin CArG-binding factor is indistinguishable from the c-fos serum response factor.  |  Boxer, LM., et al. 1989. Mol Cell Biol. 9: 515-22. PMID: 2710114
5. SRF/myocardin: a novel molecular axis regulating vascular smooth muscle cell stiffening in hypertension.  |  Lacolley, P., et al. 2017. Cardiovasc Res. 113: 120-122. PMID: 28003269
6. Regulation of cardiac myocyte cell death and differentiation by myocardin.  |  Gordon, JW. 2018. Mol Cell Biochem. 437: 119-131. PMID: 28631251
7. Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element.  |  Norman, C., et al. 1988. Cell. 55: 989-1003. PMID: 3203386
8. Myocardin-related transcription factor contributes to renal fibrosis through the regulation of extracellular microenvironment surrounding fibroblasts.  |  Yamamura, Y., et al. 2023. FASEB J. 37: e23005. PMID: 37289107
9. A shared role of the myocardin-family transcriptional coactivators in cardiomyocyte maturation.  |  Guo, Y., et al. 2023. Sci China Life Sci. 66: 2939-2942. PMID: 37574527
10. Myocardin reverses insulin resistance and ameliorates cardiomyopathy by increasing IRS-1 expression in a murine model of lipodystrophy caused by adipose deficiency of vacuolar H+-ATPase V0d1 subunit.  |  Yuan, W., et al. 2024. Theranostics. 14: 2246-2264. PMID: 38505620
11. Suppression of smooth muscle cell inflammation by myocardin-related transcription factors involves inactivation of TANK-binding kinase 1.  |  Bankell, E., et al. 2024. Sci Rep. 14: 13321. PMID: 38858497
12. Functional analysis of a growth factor-responsive transcription factor complex.  |  Hill, CS., et al. 1993. Cell. 73: 395-406. PMID: 8477450

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myocardin Antibody (E-5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Myocardin Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Myocardin Antibody (E-5) Specifications

Alternate Product Name: Myocardin

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 391-410

Epitope Target: Myocardin

Epitope Species: human

Product Reactivity/Detection: Myocardin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Myocardin Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin