MYLK Antibody (A-10): sc-515020

MYLK Antibody (A-10) is a mouse monoclonal IgM antibody that detects myosin light chain kinase (MYLK) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MYLK plays a crucial role in muscle contraction by phosphorylating myosin regulatory light chains, which facilitates the interaction between myosin and actin filaments, ultimately leading to contractile activity. This process is vital not only in smooth muscle but also in non-muscle and skeletal/cardiac muscle tissues, highlighting MYLK′s importance in various physiological functions. The MYLK gene encodes multiple isoforms, including those specific to smooth muscle and non-muscle, as well as telokin, a unique C-terminal isoform that stabilizes unphosphorylated myosin filaments exclusively in smooth muscle. The presence of different isoforms across a range of adult and fetal tissues underscores MYLK′s versatility and significance in muscle physiology. Additionally, MYLK interacts with p21-activated kinases (PAKs), with PAK1 binding leading to a reduction in MYLK activity and subsequent myosin light chain phosphorylation, illustrating the regulatory mechanisms that control muscle contraction. Anti-MYLK antibody (A-10) is an essential tool for researchers studying muscle biology and the intricate signaling pathways that govern muscle function.

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MYLK Antibody (A-10) References:

1. A genomic region encompassing a cluster of olfactory receptor genes and a myosin light chain kinase (MYLK) gene is duplicated on human chromosome regions 3q13-q21 and 3p13.  |  Brand-Arpon, V., et al. 1999. Genomics. 56: 98-110. PMID: 10036190
2. A single human myosin light chain kinase gene (MLCK; MYLK).  |  Lazar, V. and Garcia, JG. 1999. Genomics. 57: 256-67. PMID: 10198165
3. The functional myosin light chain kinase (MYLK) gene localizes with marker D3S3552 on human chromosome 3q21 in a >5-Mb yeast artificial chromosome region and is not linked to olfactory receptor genes.  |  Giorgi, D., et al. 2001. Cytogenet Cell Genet. 92: 85-8. PMID: 11306802
4. 130-kDa smooth muscle myosin light chain kinase is transcribed from a CArG-dependent, internal promoter within the mouse mylk gene.  |  Yin, F., et al. 2006. Am J Physiol Cell Physiol. 290: C1599-609. PMID: 16407417
5. A transcribed pseudogene of MYLK promotes cell proliferation.  |  Han, YJ., et al. 2011. FASEB J. 25: 2305-12. PMID: 21441351
6. Fine mapping of the myosin light chain kinase (MYLK) gene replicates the association with asthma in populations of Spanish descent.  |  Acosta-Herrera, M., et al. 2015. J Allergy Clin Immunol. 136: 1116-8.e9. PMID: 26025125
7. The ZEB1/miR-200c feedback loop regulates invasion via actin interacting proteins MYLK and TKS5.  |  Sundararajan, V., et al. 2015. Oncotarget. 6: 27083-96. PMID: 26334100
8. Lipopolysaccharide-Induced Increase in Intestinal Permeability Is Mediated by TAK-1 Activation of IKK and MLCK/MYLK Gene.  |  Nighot, M., et al. 2019. Am J Pathol. 189: 797-812. PMID: 30711488
9. Examination of Molecular Effects of MYLK Deletion in a Patient with Extensive Aortic, Carotid, and Abdominal Dissections That Underlie the Genetic Dysfunction.  |  Macklin, SK., et al. 2020. Case Rep Med. 2020: 5108052. PMID: 32655646
10. Correction to 'LncRNA MYLK antisense RNA 1 activates cell division cycle 42/Neutal Wiskott-Aldrich syndrome protein pathway via microRNA-101-5p to accelerate epithelial-to-mesenchymal transition of colon cancer cells'.  |  . 2024. Kaohsiung J Med Sci. 40: 869. PMID: 39228279

The preferred MYLK antibody is MYLK Antibody (A-8): sc-365352.