Myosin-2/MYH2 Antibody (A4.74): sc-53095

MYH2 Antibody (A4.74) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect the MYH2 protein, also known as Myosin Heavy Chain IIa, in mouse, rat, human, and rabbit tissues. MYH2 is a vital component of the sarcomeric thick filaments within skeletal muscle cells, where MYH2 interacts directly with actin (ACTA1) to generate the force necessary for muscle contraction. Additionally, MYH2 interacts with key regulatory proteins such as tropomyosin and the troponin complex, which modulate MYH2 activity in response to calcium signaling, thereby ensuring precise control of muscle contraction and relaxation cycles. Anti-MYH2 antibody (A4.74) is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP), making anti-MYH2 antibody (A4.74) a versatile tool for various experimental applications. By targeting adult skeletal muscle myosin, MYH2 monoclonal antibody (A4.74) enables detailed analysis of myosin interactions and dynamics, facilitating research into muscle fiber composition, contractile mechanics, and muscle-related diseases. Anti-MYH2 antibody (A4.74)′s ability to accurately detect MYH2 across multiple species supports advanced studies in muscle physiology and pathology. Anti-MYH2 antibody (A4.74) is recommended for scientists investigating the molecular mechanisms that underpin muscle function and development.

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Myosin-2/MYH2 Antibody (A4.74) References:

1. Mutations in MYH9 result in the May-Hegglin anomaly, and Fechtner and Sebastian syndromes. The May-Heggllin/Fechtner Syndrome Consortium.  |  Seri, M., et al. 2000. Nat Genet. 26: 103-5. PMID: 10973259
2. Human nonsyndromic hereditary deafness DFNA17 is due to a mutation in nonmuscle myosin MYH9.  |  Lalwani, AK., et al. 2000. Am J Hum Genet. 67: 1121-8. PMID: 11023810
3. Nonmuscle myosin heavy chain IIA mutations define a spectrum of autosomal dominant macrothrombocytopenias: May-Hegglin anomaly and Fechtner, Sebastian, Epstein, and Alport-like syndromes.  |  Heath, KE., et al. 2001. Am J Hum Genet. 69: 1033-45. PMID: 11590545
4. Asp1424Asn MYH9 mutation results in an unstable protein responsible for the phenotypes in May-Hegglin anomaly/Fechtner syndrome.  |  Deutsch, S., et al. 2003. Blood. 102: 529-34. PMID: 12649151
5. Kinetic mechanism of blebbistatin inhibition of nonmuscle myosin IIb.  |  Ramamurthy, B., et al. 2004. Biochemistry. 43: 14832-9. PMID: 15544354
6. Critical regions for assembly of vertebrate nonmuscle myosin II.  |  Nakasawa, T., et al. 2005. Biochemistry. 44: 174-83. PMID: 15628858
7. Basic mechanism of three-dimensional collagen fibre transport by fibroblasts.  |  Meshel, AS., et al. 2005. Nat Cell Biol. 7: 157-64. PMID: 15654332
8. Myosin 2 is a key Rho kinase target necessary for the local concentration of E-cadherin at cell-cell contacts.  |  Shewan, AM., et al. 2005. Mol Biol Cell. 16: 4531-42. PMID: 16030252
9. Human nonmuscle myosin heavy chain mRNA: generation of diversity through alternative polyadenylylation.  |  Saez, CG., et al. 1990. Proc Natl Acad Sci U S A. 87: 1164-8. PMID: 1967836
10. Myosin 18A coassembles with nonmuscle myosin 2 to form mixed bipolar filaments.  |  Billington, N., et al. 2015. Curr Biol. 25: 942-8. PMID: 25754640
11. Myosin 2-Induced Mitotic Rounding Enables Columnar Epithelial Cells to Interpret Cortical Spindle Positioning Cues.  |  Chanet, S., et al. 2017. Curr Biol. 27: 3350-3358.e3. PMID: 29107549
12. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.  |  Hughes, SM., et al. 1993. Dev Biol. 158: 183-99. PMID: 7687223

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Myosin-2/MYH2 Antibody (A4.74) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-Myosin-2/MYH2 Antibody (A4.74) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Myosin-2/MYH2 Antibody (A4.74) Specifications

Alternate Product Name: MYH2

Product Clone Name: A4.74

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: adult skeletal muscle myosin

Epitope Species: human

Product Reactivity/Detection: MYH2

Species Reactivity/Detection: mouse, rat, human, rabbit

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for Myosin-2/MYH2 Antibody (A4.74): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred antibody is Antibody (): sc-32732m.