Myosin-2/MYH2 Antibody (6D596): sc-71631

MYH2 Antibody (6D596) is a mouse monoclonal IgG1 kappa light chain antibody specifically designed to detect MYH2, also known as myosin heavy chain 2, MyHC-IIa, or fast-twitch myosin heavy chain IIa, in mouse, rat, human, and rabbit tissues. MYH2 monoclonal antibody (6D596) targets adult skeletal muscle myosin of human origin and is validated for use in western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications, ensuring reliable performance across a range of experimental techniques. MYH2 is a critical component of sarcomeric thick filaments in skeletal muscle fibers, particularly in fast-twitch (type IIa) muscle fibers, where MYH2 plays an essential role in muscle contraction by interacting with actin filaments to generate force necessary for rapid movements. Located within the A-band of the sarcomere, MYH2′s precise positioning is crucial for the sliding filament mechanism, allowing efficient cross-bridge cycling with actin in thin filaments during contraction. MYH2′s cellular localization directly influences speed and strength of muscle contraction, affecting muscle performance and adaptability. Understanding MYH2 distribution and function is vital for studying muscle physiology, fiber-type composition, and various muscle-related diseases such as myopathies and muscular dystrophies. Anti-MYH2 antibody (6D596) aids researchers in investigating molecular mechanisms underlying muscle function, adaptation, and pathology.

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Myosin-2/MYH2 Antibody (6D596) References:

1. Myopathies associated with myosin heavy chain mutations.  |  Oldfors, A., et al. 2004. Acta Myol. 23: 90-6. PMID: 15605950
2. Myosin heavy chain isoform composition and Ca(2+) transients in fibres from enzymatically dissociated murine soleus and extensor digitorum longus muscles.  |  Calderón, JC., et al. 2010. J Physiol. 588: 267-79. PMID: 19884322
3. C/EBPα represses slow myosin heavy chain 2 gene expression in developing avian myotubes.  |  Cavanaugh, EJ. and DiMario, JX. 2016. Biochim Biophys Acta. 1860: 2355-2362. PMID: 27424922
4. Myosin heavy chains in extraocular muscle fibres: Distribution, regulation and function.  |  Hoh, JFY. 2021. Acta Physiol (Oxf). 231: e13535. PMID: 32640094
5. Myosin heavy chain expression relationships to power-load and velocity-load curves.  |  Lane, MT. and Fry, AC. 2021. J Sports Med Phys Fitness. 61: 2-9. PMID: 33092319
6. Changes in myocardial myosin heavy chain isoform composition with exercise and post-exercise cold-water immersion.  |  Al-Horani, RA., et al. 2021. J Muscle Res Cell Motil. 42: 183-191. PMID: 33826086
7. Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells.  |  Chen, J., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34873039
8. A noninvasive test for estimating myosin heavy chain of the vastus lateralis in females with mechanomyography.  |  Sontag, SA., et al. 2023. Med Eng Phys. 111: 103946. PMID: 36792240
9. Human and African ape myosin heavy chain content and the evolution of hominin skeletal muscle.  |  Queeno, SR., et al. 2023. Comp Biochem Physiol A Mol Integr Physiol. 281: 111415. PMID: 36931425
10. C-terminal isoforms of the myosin heavy chain and smooth muscle function.  |  Martin, AF., et al. 1997. Comp Biochem Physiol B Biochem Mol Biol. 117: 3-11. PMID: 9180009

The preferred antibody is Antibody (): sc-32732m.