Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MYG1 CRISPR/Cas9 KO Plasmid (h): sc-405754
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MYG1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MYG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MYG1 Antibody (F-5): sc-393331
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MYG1 CRISPR/Cas9 KO Plasmid (h) | sc-405754 | 20 µg | $397.00 |
Overview
C12orf10 encodes MYG1, a conserved nuclease implicated in RNA metabolism and genome maintenance, with reported activity toward RNA substrates and roles in mitochondrial and nuclear RNA processing. MYG1 has been linked to regulation of mitochondrial gene expression and cellular energy homeostasis, connecting it to pathways that coordinate RNA turnover, oxidative phosphorylation, and stress-adaptive transcriptional programs. Perturbation of MYG1 function can disrupt RNA quality control and organelle function, processes frequently associated with altered proliferation, proteostasis imbalance, and inflammatory signaling in disease-relevant contexts. These features make MYG1 a useful target for mechanistic studies of RNA processing networks and mitochondrial–nuclear communication in human cells.
MYG1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the C12orf10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C12orf10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C12orf10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MYG1 protein expression.
This CRISPR knockout system enables efficient generation of C12orf10-deficient cell models for investigation of MYG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C12orf10 exon(s) critical for MYG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C12orf10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MYG1 CRISPR/Cas9 KO Plasmid (h) and MYG1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the C12orf10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MYG1 HDR Plasmid (h) and MYG1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by C12orf10 homology arms to support homology-directed repair at defined C12orf10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.