c-Myc Antibody (C-8): sc-41. Western blot analysis of c-Myc expression in non-transfected: sc-117752 (A) and mouse c-Myc transfected: sc-118892 (B) 293T whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
c-Myc Antibody (C-8): sc-41. Western blot analysis of c-Myc expression in non-transfected: sc-117752 (A) and mouse c-Myc transfected: sc-118892 (B) 293T whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
Myc CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Myc genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Myc Antibody (9E10): sc-40