Mx1 CRISPR/Cas9 KO Plasmid (m): sc-421763
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mx1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Mx1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mx1 Antibody (E-5): sc-271024
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mx1 CRISPR/Cas9 KO Plasmid (m) | sc-421763 | 20 µg | $397.00 |
Overview
Mx1 (myxovirus resistance 1) encodes a dynamin-like interferon-stimulated GTPase that functions as a key effector of the innate antiviral response in mouse cells. Following type I and type III interferon signaling through the JAK–STAT pathway, Mx1 is transcriptionally induced and can restrict replication of a range of RNA viruses by disrupting viral nucleocapsid trafficking and replication complex formation. Mx1 activity intersects with broader interferon-stimulated gene (ISG) networks that shape inflammatory signaling, antigen presentation, and cell-intrinsic immunity. Variation in Mx1-dependent restriction has been leveraged to study host susceptibility to viral infection, interferon-driven immunopathology, and mechanisms of immune evasion.
Mx1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mx1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mx1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mx1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Mx1 protein expression.
This CRISPR knockout system enables efficient generation of Mx1-deficient cell models for investigation of Mx1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mx1 exon(s) critical for Mx1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mx1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Mx1 CRISPR/Cas9 KO Plasmid (m) and Mx1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mx1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Mx1 HDR Plasmid (m) and Mx1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mx1 homology arms to support homology-directed repair at defined Mx1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.