Munc13-4 Antibody (C-2): sc-271300
- Munc13-4 Antibody (C-2) is a mouse monoclonal IgG1 κ Munc13-4 antibody provided at 200 µg/ml
- raised against amino acids 136-285 mapping near the N-terminus of Munc13-4 of human origin
- Munc13-4 Antibody (C-2) is recommended for detection of Munc13-4 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Munc13-4 Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Munc13-4 Antibody (C-2) for WB and IHC(P) applications. These reagents are now offered in bundles with Munc13-4 Antibody (C-2) (see ordering information below).
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Munc13-4 Antibody (C-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Munc13-4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Munc13-4 (C-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Munc13-4 is a crucial member of the Munc13 family, sharing homology with Munc13-1, but notably lacking the C1 domain and N-terminal extension found in other family members. Munc13-4 plays a significant role in regulating secretory lysosome fusion and exocytosis, particularly in immune cells such as cytotoxic T lymphocytes and mast cells, where Munc13-4 enhances the degranulation process. Munc13-4 is primarily localized to secretory lysosomes, which are essential for the release of cytotoxic granules in immune responses. Expression of Munc13-4 is predominantly observed in mucous goblet and alveolar type II cells of the lung, highlighting its importance in respiratory health. Mutations in Munc13-4 are linked to familial hemophagocytic lymphohistiocytosis subtype 3, underscoring its critical role in immune system function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Munc13-4 Antibody (C-2) References:
- Munc13-4 is essential for cytolytic granules fusion and is mutated in a form of familial hemophagocytic lymphohistiocytosis (FHL3). | Feldmann, J., et al. 2003. Cell. 115: 461-73. PMID: 14622600
- Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets. | Shirakawa, R., et al. 2004. J Biol Chem. 279: 10730-7. PMID: 14699162
- Munc13-4 is an effector of rab27a and controls secretion of lysosomes in hematopoietic cells. | Neeft, M., et al. 2005. Mol Biol Cell. 16: 731-41. PMID: 15548590
- Genetic subtypes of familial hemophagocytic lymphohistiocytosis: correlations with clinical features and cytotoxic T lymphocyte/natural killer cell functions. | Ishii, E., et al. 2005. Blood. 105: 3442-8. PMID: 15632205
- Mutations of syntaxin 11 and SNAP23 genes as causes of familial hemophagocytic lymphohistiocytosis were not found in Japanese people. | Yamamoto, K., et al. 2005. J Hum Genet. 50: 600-603. PMID: 16180048
- Cytotoxic T lymphocyte exocytosis: bring on the SNAREs! | Hong, W. 2005. Trends Cell Biol. 15: 644-50. PMID: 16260137
- Correlation between phenotypic heterogeneity and gene mutational characteristics in familial hemophagocytic lymphohistiocytosis (FHL). | Ueda, I., et al. 2006. Pediatr Blood Cancer. 46: 482-8. PMID: 16365863
- Mutations of perforin and Munc13-4 do not mark HLH by NK defects. | Schneider, EM. 2006. Pediatr Blood Cancer. 46: 409-11. PMID: 16365870
- Familial hemophagocytic lymphohistiocytosis with the MUNC13-4 mutation: a case report. | Mizumoto, H., et al. 2006. Eur J Pediatr. 165: 384-8. PMID: 16416131
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Munc13-4 Antibody (C-2) | sc-271300 | 200 µg/ml | $316.00 | |||
Munc13-4 Antibody (C-2): m-IgG Fc BP-HRP Bundle | sc-529158 | 200 µg Ab; 10 µg BP | $354.00 | |||
Munc13-4 Antibody (C-2): m-IgGκ BP-HRP Bundle | sc-521904 | 200 µg Ab, 40 µg BP | $354.00 | |||
Munc13-4 Antibody (C-2) AC | sc-271300 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Munc13-4 Antibody (C-2) HRP | sc-271300 HRP | 200 µg/ml | $316.00 | |||
Munc13-4 Antibody (C-2) FITC | sc-271300 FITC | 200 µg/ml | $330.00 | |||
Munc13-4 Antibody (C-2) PE | sc-271300 PE | 200 µg/ml | $343.00 | |||
Munc13-4 Antibody (C-2) Alexa Fluor® 488 | sc-271300 AF488 | 200 µg/ml | $357.00 | |||
Munc13-4 Antibody (C-2) Alexa Fluor® 546 | sc-271300 AF546 | 200 µg/ml | $357.00 | |||
Munc13-4 Antibody (C-2) Alexa Fluor® 594 | sc-271300 AF594 | 200 µg/ml | $357.00 | |||
Munc13-4 Antibody (C-2) Alexa Fluor® 647 | sc-271300 AF647 | 200 µg/ml | $357.00 | |||
Munc13-4 Antibody (C-2) Alexa Fluor® 680 | sc-271300 AF680 | 200 µg/ml | $357.00 | |||
Munc13-4 Antibody (C-2) Alexa Fluor® 790 | sc-271300 AF790 | 200 µg/ml | $357.00 |