MSY2 CRISPR/Cas9 KO Plasmid (h): sc-405504
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MSY2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MSY2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MSY2 Antibody (A-12): sc-393840
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MSY2 CRISPR/Cas9 KO Plasmid (h) | sc-405504 | 20 µg | $397.00 |
Overview
YBX2 encodes MSY2, a conserved Y-box RNA/DNA-binding protein that recognizes Y-box motifs and regulates post-transcriptional gene expression through mRNA stabilization, storage, and translational control. MSY2 is best known for roles in germ-cell biology and spermatogenesis, where it contributes to mRNP assembly and timing of protein synthesis during meiotic and post-meiotic differentiation. In somatic contexts, Y-box family proteins interface with RNA processing, stress response programs, and cell state transitions that influence proliferation and survival. Dysregulated YBX2 expression has been linked to impaired male fertility and has been reported in transcriptional signatures associated with tumor biology, supporting its use as a mechanistic target for studying RNA-centric regulatory pathways.
MSY2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the YBX2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the YBX2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the YBX2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MSY2 protein expression.
This CRISPR knockout system enables efficient generation of YBX2-deficient cell models for investigation of MSY2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting YBX2 exon(s) critical for MSY2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple YBX2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MSY2 CRISPR/Cas9 KO Plasmid (h) and MSY2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the YBX2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MSY2 HDR Plasmid (h) and MSY2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by YBX2 homology arms to support homology-directed repair at defined YBX2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.