Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MSL3L1 CRISPR/Cas9 KO Plasmid (h): sc-407378
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MSL3L1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MSL3L1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MSL3L1 Antibody (E-7): sc-518210
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MSL3L1 CRISPR/Cas9 KO Plasmid (h) | sc-407378 | 20 µg | $397.00 |
Overview
MSL3 encodes MSL3L1, a chromatin-associated protein implicated in epigenetic regulation through interactions with histone acetylation machinery and transcriptional control complexes. MSL3L1 is linked to modulation of chromatin accessibility and gene expression programs that influence cell cycle progression, differentiation, and genome stability. As part of broader histone modification networks, MSL3L1 supports coordinated regulation of transcriptional output in response to developmental and environmental cues. Dysregulation of chromatin regulators in this pathway is frequently associated with altered transcriptional states observed in cancer biology and neurodevelopmental research contexts.
MSL3L1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MSL3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MSL3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MSL3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MSL3L1 protein expression.
This CRISPR knockout system enables efficient generation of MSL3-deficient cell models for investigation of MSL3L1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MSL3 exon(s) critical for MSL3L1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MSL3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MSL3L1 CRISPR/Cas9 KO Plasmid (h) and MSL3L1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MSL3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MSL3L1 HDR Plasmid (h) and MSL3L1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MSL3 homology arms to support homology-directed repair at defined MSL3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.