mSin3A Antibody (G-11): sc-5299

mSin3A Antibody (G-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects mSin3A of mouse, rat, and human origin through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). mSin3A (G-11) antibody is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. mSin3A plays a crucial role in the regulation of gene expression, particularly in cell proliferation and differentiation, by functioning as a corepressor in transcriptional regulation. mSin3A interacts with the Mad family of proteins, which are essential for the repression of target genes when bound to the Myc-Max heterodimer. This interaction helps maintain the balance between cell growth and differentiation, preventing uncontrolled proliferation that can lead to tumorigenesis. mSin3A contains four conserved paired amphipathic helix (PAH) domains, which are important for interaction with Mad proteins, specifically through the second PAH domain (PAH2). By tethering mSin3A to DNA, the Mad-Max heterodimers effectively repress transcription, highlighting mSin3A′s importance in cellular processes and potential implications in cancer biology.

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mSin3A Antibody (G-11) References:

1. Myc and Max proteins possess distinct transcriptional activities.  |  Kretzner, L., et al. 1992. Nature. 359: 426-9. PMID: 1406956
2. The mSin3A chromatin-modifying complex is essential for embryogenesis and T-cell development.  |  Cowley, SM., et al. 2005. Mol Cell Biol. 25: 6990-7004. PMID: 16055712
3. Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants.  |  Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-92. PMID: 1644290
4. Repression of p53-mediated transcription by adenovirus E1B 55-kDa does not require corepressor mSin3A and histone deacetylases.  |  Zhao, LY., et al. 2007. J Biol Chem. 282: 7001-10. PMID: 17209038
5. Methylglyoxal modification of mSin3A links glycolysis to angiopoietin-2 transcription.  |  Yao, D., et al. 2007. Cell. 128: 625. PMID: 17387784
6. Exercise and diabetes have opposite effects on the assembly and O-GlcNAc modification of the mSin3A/HDAC1/2 complex in the heart.  |  Cox, EJ. and Marsh, SA. 2013. Cardiovasc Diabetol. 12: 101. PMID: 23835259
7. Transcriptional repression of Aurora-A gene by wild-type p53 through directly binding to its promoter with histone deacetylase 1 and mSin3a.  |  Yang, TY., et al. 2018. Int J Cancer. 142: 92-108. PMID: 28884479
8. Mad-Max transcriptional repression is mediated by ternary complex formation with mammalian homologs of yeast repressor Sin3.  |  Ayer, DE., et al. 1995. Cell. 80: 767-76. PMID: 7889570
9. An amino-terminal domain of Mxi1 mediates anti-Myc oncogenic activity and interacts with a homolog of the yeast transcriptional repressor SIN3.  |  Schreiber-Agus, N., et al. 1995. Cell. 80: 777-86. PMID: 7889571
10. The c-Myc protein induces cell cycle progression and apoptosis through dimerization with Max.  |  Amati, B., et al. 1993. EMBO J. 12: 5083-7. PMID: 8262051
11. Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity.  |  Ayer, DE., et al. 1993. Cell. 72: 211-22. PMID: 8425218
12. Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation.  |  Hurlin, PJ., et al. 1996. EMBO J. 15: 2030. PMID: 8617250

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-mSin3A Antibody (G-11) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-mSin3A Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

mSin3A Antibody (G-11) Specifications

Alternate Product Name: mSin3A

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: mSin3A

Epitope Species: mouse

Product Reactivity/Detection: mSin3A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for mSin3A Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent