Msi1 Antibody (69-Q): sc-135721
- Msi1 Antibody (69-Q) is a mouse monoclonal IgG2a κ Msi1 antibody, cited in 11 publications, provided at 100 µg/ml
- raised against amino acids 1-87 representing an N-terminal region of Msi1 of human origin
- recommended for detection of Msi1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Msi1 Antibody (69-Q) for WB and IHC(P) applications. These reagents are now offered in bundles with Msi1 Antibody (69-Q) (see ordering information below).
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Msi1 Antibody (69-Q) is a mouse monoclonal IgG2a kappa light chain antibody that detects Msi1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Msi1 antibody (69-Q) is available as the non-conjugated form. Musashi1 (Msi1) is a crucial RNA-binding protein predominantly expressed in neural progenitor cells and neural stem cells, playing a significant role in the regulation of cell fate during development. The gene encoding human Msi1 produces a 362 amino acid protein that is essential for maintaining the balance between self-renewal and differentiation of stem cells in the central nervous system. Msi1 (69-Q) antibody localizes to the cytoplasm in murine embryonic neural progenitor cells and shows decreased expression as these cells differentiate, highlighting its importance in the early stages of neural development. Msi1 interacts with NUMB, a protein that serves as a membrane-associated antagonist of Notch signaling, thereby influencing critical pathways involved in cell proliferation and differentiation. Beyond serving as a marker for neural progenitor cells in healthy human brains, Msi1 is also implicated in human gliomas, making Msi1 a valuable target for cancer research. Msi1 expression extends to Sertoli cells of the testis and granulosa cells of the ovary in rats, underscoring Msi1′s diverse biological significance across different tissues.
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Msi1 Antibody (69-Q) References:
- Musashi1: an evolutionally conserved marker for CNS progenitor cells including neural stem cells. | Kaneko, Y., et al. 2000. Dev Neurosci. 22: 139-53. PMID: 10657706
- The neural RNA-binding protein Musashi1 translationally regulates mammalian numb gene expression by interacting with its mRNA. | Imai, T., et al. 2001. Mol Cell Biol. 21: 3888-900. PMID: 11359897
- Musashi1, an evolutionarily conserved neural RNA-binding protein, is a versatile marker of human glioma cells in determining their cellular origin, malignancy, and proliferative activity. | Kanemura, Y., et al. 2001. Differentiation. 68: 141-52. PMID: 11686236
- RNA binding protein Musashi1 is expressed in sertoli cells in the rat testis from fetal life to adulthood. | Saunders, PT., et al. 2002. Biol Reprod. 66: 500-7. PMID: 11804968
- RNA-binding protein Musashi family: roles for CNS stem cells and a subpopulation of ependymal cells revealed by targeted disruption and antisense ablation. | Sakakibara, S., et al. 2002. Proc Natl Acad Sci U S A. 99: 15194-9. PMID: 12407178
- The human Musashi homolog 1 (MSI1) gene encoding the homologue of Musashi/Nrp-1, a neural RNA-binding protein putatively expressed in CNS stem cells and neural progenitor cells. | Good, P., et al. 1998. Genomics. 52: 382-4. PMID: 9790759
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Msi1 Antibody (69-Q) | sc-135721 | 100 µg/ml | $333.00 | |||
Msi1 Antibody (69-Q): m-IgG Fc BP-HRP Bundle | sc-537370 | 100 µg Ab; 10 µg BP | $354.00 | |||
Msi1 Antibody (69-Q): m-IgGκ BP-HRP Bundle | sc-534618 | 100 µg Ab; 40 µg BP | $354.00 | |||
Msi1 Antibody (69-Q): m-IgG2a BP-HRP Bundle | sc-547998 | 100 µg Ab; 10 µg BP | $354.00 |