MSG1 Antibody (D-7): sc-393585

MSG1 Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects MSG1 of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MSG1 (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MSG1, also known as CITED1, plays a crucial role in gene expression regulation, particularly in melanocytes, where MSG1 is involved in transcriptional activation of pigmentation-related genes. This function helps maintain melanocyte differentiation, essential for normal skin pigmentation and UV radiation protection. MSG1 expression is significantly higher in strongly pigmented melanoma cells compared to weakly pigmented cells, indicating MSG1′s potential role in melanoma progression and pigmentation regulation. MSG1 is localized in the nucleus and specifically expressed in melanocytes and testis, with human MSG1 sharing 75% sequence homology with mouse MSG1. Additionally, MSG1 features a serine/threonine-rich region, and studies have shown that a fusion protein containing MSG1 and a DNA-binding domain can activate transcription in mammalian cells, with this activation depending on MSG1′s acidic domain.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MSG1 Antibody (D-7) References:

1. Expression of genes for microphthalmia isoforms, Pax3 and MSG1, in human melanomas.  |  Vachtenheim, J. and Novotná, H. 1999. Cell Mol Biol (Noisy-le-grand). 45: 1075-82. PMID: 10644012
2. The MSG1 non-DNA-binding transactivator binds to the p300/CBP coactivators, enhancing their functional link to the Smad transcription factors.  |  Yahata, T., et al. 2000. J Biol Chem. 275: 8825-34. PMID: 10722728
3. Aberrant expression of MSG1 transcriptional activator in human malignant melanoma in vivo.  |  Ahmed, NU., et al. 2001. Pigment Cell Res. 14: 140-3. PMID: 11310794
4. Over-expression of MSG1 transcriptional co-activator increases melanin in B16 melanoma cells: a possible role for MSG1 in melanogenesis.  |  Nair, SS., et al. 2001. Pigment Cell Res. 14: 206-9. PMID: 11434569
5. Cited1 is required in trophoblasts for placental development and for embryo growth and survival.  |  Rodriguez, TA., et al. 2004. Mol Cell Biol. 24: 228-44. PMID: 14673158
6. CITED1 protein expression suggests Papillary Thyroid Carcinoma in high throughput tissue microarray-based study.  |  Prasad, ML., et al. 2004. Thyroid. 14: 169-75. PMID: 15072698
7. Galectin-3, fibronectin-1, CITED-1, HBME1 and cytokeratin-19 immunohistochemistry is useful for the differential diagnosis of thyroid tumors.  |  Prasad, ML., et al. 2005. Mod Pathol. 18: 48-57. PMID: 15272279
8. Cited1 is a bifunctional transcriptional cofactor that regulates early nephronic patterning.  |  Plisov, S., et al. 2005. J Am Soc Nephrol. 16: 1632-44. PMID: 15843474
9. msg1, a novel melanocyte-specific gene, encodes a nuclear protein and is associated with pigmentation.  |  Shioda, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 12298-303. PMID: 8901575
10. The MSG1 and AXR1 genes of Arabidopsis are likely to act independently in growth-curvature responses of hypocotyls.  |  Watahiki, MK., et al. 1999. Planta. 207: 362-9. PMID: 9951732

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MSG1 Antibody (D-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-MSG1 Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MSG1 Antibody (D-7) Specifications

Alternate Product Name: MSG1

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-167

Epitope Target: MSG1

Epitope Species: mouse

Product Reactivity/Detection: MSG1

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for MSG1 Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin