MRTF-A/MKL1 Antibody (G-7): sc-398675

MRTF-A Antibody (G-7) is a mouse monoclonal IgM antibody that detects MRTF-A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MRTF-A antibody (G-7) is available as both non-conjugated and multiple Alexa Fluor® conjugates. The myocardin-related transcription factor A (MRTF-A), also known as MKL1, plays a crucial role in cellular signaling and gene expression, particularly in muscle cells. MRTF-A (G-7) antibody primarily localizes in the cytoplasm under resting conditions but translocates to the nucleus in response to growth factors and mechanical stress, where MRTF-A interacts with serum response factor (SRF) to regulate genes involved in muscle development and cardiovascular function. This translocation allows MRTF-A to modulate SRF transcriptional activity, influencing genes necessary for muscle differentiation and proliferation. MRTF-A shuttling between cytoplasm and nucleus ensures appropriate gene activation in response to physiological stimuli, making MRTF-A essential for cardiovascular system development and maintenance.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MRTF-A/MKL1 Antibody (G-7) References:

1. Regulation of MRTF-A by JMY via a nucleation-independent mechanism.  |  Kluge, F., et al. 2018. Cell Commun Signal. 16: 86. PMID: 30463620
2. MRTF-A regulates proliferation and survival properties of pro-atherogenic macrophages.  |  An, J., et al. 2019. J Mol Cell Cardiol. 133: 26-35. PMID: 31128166
3. A cAbl-MRTF-A Feedback Loop Contributes to Hepatic Stellate Cell Activation.  |  Lu, Y., et al. 2019. Front Cell Dev Biol. 7: 243. PMID: 31681772
4. An MRTF-A-Sp1-PDE5 Axis Mediates Angiotensin-II-Induced Cardiomyocyte Hypertrophy.  |  Wu, T., et al. 2020. Front Cell Dev Biol. 8: 839. PMID: 33015041
5. MRTF-A regulates Ca2+ release through CACNA1S.  |  Liang, C., et al. 2021. J Biosci. 46: PMID: 33969828
6. MRTF-A regulates myoblast commitment to differentiation by targeting PAX7 during muscle regeneration.  |  Song, R., et al. 2021. J Cell Mol Med. 25: 8645-8661. PMID: 34347392
7. Inhibition of RhoA/MRTF-A signaling alleviates nucleus pulposus fibrosis induced by mechanical stress overload.  |  Song, M., et al. 2022. Connect Tissue Res. 63: 53-68. PMID: 34420462
8. SIRT6 mediates MRTF-A deacetylation in vascular endothelial cells to antagonize oxLDL-induced ICAM-1 transcription.  |  Huang, S., et al. 2022. Cell Death Discov. 8: 96. PMID: 35246513
9. MRTF-A alleviates myocardial ischemia reperfusion injury by inhibiting the inflammatory response and inducing autophagy.  |  Zhong, Z., et al. 2023. Mol Cell Biochem. 478: 343-359. PMID: 35829871
10. MBNL1 and MRTF-A form a positive feedback loop in regulating the migration of esophageal cancer cells.  |  Liang, C., et al. 2022. J Cancer Res Ther. 18: 1312-1319. PMID: 36204878

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MRTF-A/MKL1 Antibody (G-7) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-MRTF-A/MKL1 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MRTF-A/MKL1 Antibody (G-7) Specifications

Alternate Product Name: MKL1

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 905-931

Epitope Target: MRTF-A

Epitope Species: human

Product Reactivity/Detection: MRTF-A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for MRTF-A/MKL1 Antibody (G-7): P: Blocking Peptide

The preferred MRTF-A/MKL1 antibody is MRTF-A/MKL1 Antibody (G-8): sc-390324.