MRRF Antibody (J-Q7): sc-100969

MRRF Antibody (J-Q7) is a mouse monoclonal IgG2a kappa light chain antibody that detects MRRF protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-MRRF antibody (J-Q7) is available as the non-conjugated format. MRRF (mitochondrial ribosome recycling factor) is a crucial 262 amino acid protein that plays a significant role in the termination of protein synthesis within mitochondria. MRRF (J-Q7) antibody enables detection of a protein essential for releasing ribosomes from messenger RNA at the conclusion of protein biosynthesis, ensuring ribosome recycling for subsequent rounds of translation. This recycling process maintains translational efficiency, directly influencing protein synthesis rates critical for cellular function and energy production. MRRF exists in multiple isoforms due to alternative splicing, contributing to functional diversity and regulatory mechanisms within mitochondrial protein synthesis. Anti-MRRF antibody (J-Q7) enables researchers to gain valuable insights into mitochondrial function and protein synthesis processes in human cells.

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MRRF Antibody (J-Q7) References:

1. Assignment of the human mitochondrial translational release factor 1 (MTRF1) to chromosome 13q14.1-->q14.3 and of the human mitochondrial ribosome recycling factor (MRRF) to chromosome 9q32-->q34.1 with radiation hybrid mapping.  |  Hansen, LL., et al. 2000. Cytogenet Cell Genet. 88: 91-2. PMID: 10773675
2. Binding of ribosome recycling factor to ribosomes, comparison with tRNA.  |  Hirokawa, G., et al. 2002. J Biol Chem. 277: 35847-52. PMID: 12138121
3. The role of ribosome recycling factor in dissociation of 70S ribosomes into subunits.  |  Hirokawa, G., et al. 2005. RNA. 11: 1317-28. PMID: 16043510
4. Progression of the ribosome recycling factor through the ribosome dissociates the two ribosomal subunits.  |  Barat, C., et al. 2007. Mol Cell. 27: 250-261. PMID: 17643374
5. Crystal structure of the ribosome recycling factor bound to the ribosome.  |  Weixlbaumer, A., et al. 2007. Nat Struct Mol Biol. 14: 733-7. PMID: 17660830
6. Recycling of eukaryotic posttermination ribosomal complexes.  |  Pisarev, AV., et al. 2007. Cell. 131: 286-99. PMID: 17956730
7. Structural Insights into ribosome recycling factor interactions with the 70S ribosome.  |  Pai, RD., et al. 2008. J Mol Biol. 376: 1334-47. PMID: 18234219
8. Quantitation of the hydroxycinnamic acid derivatives and the glycosides of flavonols and flavones by UV absorbance after identification by LC-MS.  |  Lin, LZ., et al. 2012. J Agric Food Chem. 60: 544-53. PMID: 22136064
9. Structural insights into unique features of the human mitochondrial ribosome recycling.  |  Koripella, RK., et al. 2019. Proc Natl Acad Sci U S A. 116: 8283-8288. PMID: 30962385
10. International Atherosclerosis Society Roadmap for Familial Hypercholesterolaemia.  |  Watts, GF., et al. 2024. Glob Heart. 19: 12. PMID: 38273993
11. Assessment of human motor unit twitches--a comparison of spike-triggered averaging and intramuscular microstimulation.  |  Kossev, A., et al. 1994. Electroencephalogr Clin Neurophysiol. 93: 100-5. PMID: 7512915
12. Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor.  |  Zhang, Y. and Spremulli, LL. 1998. Biochim Biophys Acta. 1443: 245-50. PMID: 9838146

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MRRF Antibody (J-Q7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-MRRF Antibody (J-Q7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MRRF Antibody (J-Q7) Specifications

Alternate Product Name: MRRF

Product Clone Name: J-Q7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 163-263

Epitope Target: MRRF

Epitope Species: human

Product Reactivity/Detection: MRRF

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for MRRF Antibody (J-Q7): N/A