MRP6 CRISPR/Cas9 KO Plasmid (h): sc-402219
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MRP6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MRP6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MRP6 Antibody (M6II-31): sc-59618
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP6 CRISPR/Cas9 KO Plasmid (h) | sc-402219 | 20 µg | $397.00 |
Overview
ABCC6 encodes the ATP-binding cassette transporter MRP6, a plasma membrane efflux pump predominantly expressed in liver and kidney that regulates extracellular nucleotide and metabolite homeostasis. MRP6 activity influences purinergic signaling and inorganic pyrophosphate balance, linking transporter function to pathways that control ectopic mineralization and connective tissue integrity. Loss-of-function variants in ABCC6 are associated with pseudoxanthoma elasticum and related calcification phenotypes, making it a relevant target for studying extracellular matrix remodeling, vascular calcification, and tissue-specific transporter biology. ABCC6/MRP6 models also support investigation of ABC transporter trafficking, ATP-dependent substrate transport, and compensatory pathways among multidrug resistance proteins.
MRP6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ABCC6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ABCC6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ABCC6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MRP6 protein expression.
This CRISPR knockout system enables efficient generation of ABCC6-deficient cell models for investigation of MRP6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ABCC6 exon(s) critical for MRP6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ABCC6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MRP6 CRISPR/Cas9 KO Plasmid (h) and MRP6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ABCC6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MRP6 HDR Plasmid (h) and MRP6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ABCC6 homology arms to support homology-directed repair at defined ABCC6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.