MRP3 Antibody (B-2): sc-518159
- MRP3 Antibody (B-2) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 880-900 of MRP3 of mouse origin
- recommended for detection of MRP3 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-MRP3 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG3 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MRP3 Antibody (B-2) for WB applications. These reagents are now offered in bundles with MRP3 Antibody (B-2) (see ordering information below).
MRP3 Antibody (B-2) is a mouse monoclonal IgG3 kappa light chain antibody that detects MRP3 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MRP3 (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MRP3, a member of the ATP-binding cassette (ABC) transporter family, plays a crucial role in the cellular export of organic anions and is particularly important in the context of drug resistance in cancer therapy. MRP3 is primarily located in the basolateral membrane of hepatocytes, where MRP3 facilitates the transport of conjugated metabolites, such as S-(2,4-dinitrophenyl-) glutathione, from the cytoplasm to the bloodstream. MRP3 localization is significant because MRP3 allows for the efficient clearance of potentially harmful substances from the liver, especially when the apical transporter MRP2 is compromised. Under normal physiological conditions, MRP3 is expressed at lower levels compared to MRP2, but MRP3 expression can be upregulated in response to cellular stress or impaired secretion, highlighting MRP3′s adaptive role in maintaining hepatic function and drug metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MRP3 Antibody (B-2) References:
- MRP3, an organic anion transporter able to transport anti-cancer drugs. | Kool, M., et al. 1999. Proc Natl Acad Sci U S A. 96: 6914-9. PMID: 10359813
- Mrp3, a mitogen-regulated protein/proliferin gene expressed in wound healing and in hair follicles. | Fassett, JT. and Nilsen-Hamilton, M. 2001. Endocrinology. 142: 2129-37. PMID: 11316781
- Identification and functional characterization of the natural variant MRP3-Arg1297His of human multidrug resistance protein 3 (MRP3/ABCC3). | Lee, YM., et al. 2004. Pharmacogenetics. 14: 213-23. PMID: 15083066
- Mice lacking Mrp3 (Abcc3) have normal bile salt transport, but altered hepatic transport of endogenous glucuronides. | Zelcer, N., et al. 2006. J Hepatol. 44: 768-75. PMID: 16225954
- MRP3 gene expression correlates with NRF2 mutations in lung squamous cell carcinomas. | Sasaki, H., et al. 2012. Mol Med Rep. 6: 705-8. PMID: 22767273
- Identification of novel MRP3 inhibitors based on computational models and validation using an in vitro membrane vesicle assay. | Ali, I., et al. 2017. Eur J Pharm Sci. 103: 52-59. PMID: 28238947
- Therapy-relevant aberrant expression of MRP3 and BCRP mRNA in TCC-/SCC-bladder cancer tissue of untreated patients. | Rady, M., et al. 2017. Oncol Rep. 38: 551-560. PMID: 28586062
- MRP3 is a sex determining gene in the diatom Pseudo-nitzschia multistriata. | Russo, MT., et al. 2018. Nat Commun. 9: 5050. PMID: 30487611
- MRP3-Mediated Chemoresistance in Cholangiocarcinoma: Target for Chemosensitization Through Restoring SOX17 Expression. | Lozano, E., et al. 2020. Hepatology. 72: 949-964. PMID: 31863486
- Influence of MRP3 Genetics and Hepatic Expression Ontogeny for Morphine Disposition in Neonatal and Pediatric Patients. | Hahn, D., et al. 2020. J Clin Pharmacol. 60: 992-998. PMID: 32090339
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRP3 Antibody (B-2) | sc-518159 | 200 µg/ml | $322.00 | |||
MRP3 Antibody (B-2): m-IgGκ BP-HRP Bundle | sc-550667 | 200 µg Ab; 40 µg BP | $361.00 | |||
MRP3 Antibody (B-2): m-IgG3 BP-HRP Bundle | sc-550677 | 200 µg Ab; 40 µg BP | $361.00 | |||
MRP3 Antibody (B-2) AC | sc-518159 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MRP3 Antibody (B-2) HRP | sc-518159 HRP | 200 µg/ml | $322.00 | |||
MRP3 Antibody (B-2) FITC | sc-518159 FITC | 200 µg/ml | $336.00 | |||
MRP3 Antibody (B-2) PE | sc-518159 PE | 200 µg/ml | $349.00 | |||
MRP3 Antibody (B-2) Alexa Fluor® 488 | sc-518159 AF488 | 200 µg/ml | $364.00 | |||
MRP3 Antibody (B-2) Alexa Fluor® 546 | sc-518159 AF546 | 200 µg/ml | $364.00 | |||
MRP3 Antibody (B-2) Alexa Fluor® 594 | sc-518159 AF594 | 200 µg/ml | $364.00 | |||
MRP3 Antibody (B-2) Alexa Fluor® 647 | sc-518159 AF647 | 200 µg/ml | $364.00 | |||
MRP3 Antibody (B-2) Alexa Fluor® 680 | sc-518159 AF680 | 200 µg/ml | $364.00 | |||
MRP3 Antibody (B-2) Alexa Fluor® 790 | sc-518159 AF790 | 200 µg/ml | $364.00 |