MRP-L50 Antibody (H-10): sc-390487

MRP-L50 Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects MRP-L50 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MRP-L50 antibody (H-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MRP-L50, or mitochondrial ribosomal protein L50, is a crucial 158 amino acid protein that plays a significant role in mitochondrial protein synthesis by forming part of the 39S ribosomal subunit. MRP-L50′s localization within the mitochondrion is vital, as MRP-L50 collaborates with other mitochondrial ribosomal proteins to ensure accurate translation of mitochondrial mRNA into functional proteins, which are essential for cellular energy production and metabolism. The gene encoding MRP-L50 is located on human chromosome 9, a region rich in genes and associated with various genetic disorders, including hereditary hemorrhagic telangiectasia and familial dysautonomia. Understanding MRP-L50′s function and importance is critical, as disruptions in mitochondrial protein synthesis can lead to severe metabolic dysfunctions and contribute to various mitochondrial diseases.

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MRP-L50 Antibody (H-10) References:

1. Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria.  |  Suzuki, T., et al. 2001. J Biol Chem. 276: 21724-36. PMID: 11279069
2. The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders.  |  Kenmochi, N., et al. 2001. Genomics. 77: 65-70. PMID: 11543634
3. Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components.  |  Gan, X., et al. 2002. Eur J Biochem. 269: 5203-14. PMID: 12392552
4. DNA sequence and analysis of human chromosome 9.  |  Humphray, SJ., et al. 2004. Nature. 429: 369-74. PMID: 15164053
5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).  |  Gerhard, DS., et al. 2004. Genome Res. 14: 2121-7. PMID: 15489334
6. Nuclear MRP genes and mitochondrial disease.  |  O'Brien, TW., et al. 2005. Gene. 354: 147-51. PMID: 15908146
7. Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.  |  Yu, LR., et al. 2007. J Proteome Res. 6: 4150-62. PMID: 17924679
8. Comprehensive proteomic analysis of the effects of purine analogs on human Raji B-cell lymphoma.  |  Mactier, S., et al. 2011. J Proteome Res. 10: 1030-42. PMID: 21182289
9. Whole transcriptome sequencing reveals biologically significant RNA markers and related regulating biological pathways in cardiomyocyte hypertrophy induced by high glucose.  |  Meng, Z., et al. 2019. J Cell Biochem. 120: 1018-1027. PMID: 30242883
10. Expression analysis of mammalian mitochondrial ribosomal protein genes.  |  Cheong, A., et al. 2020. Gene Expr Patterns. 38: 119147. PMID: 32987154
11. Deficiency of the mitochondrial ribosomal subunit, MRPL50, causes autosomal recessive syndromic premature ovarian insufficiency.  |  Bakhshalizadeh, S., et al. 2023. Hum Genet. 142: 879-907. PMID: 37148394
12. Mitochondrial ribosomal proteins (MRPs) of yeast.  |  Graack, HR. and Wittmann-Liebold, B. 1998. Biochem J. 329 (Pt 3): 433-48. PMID: 9445368

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MRP-L50 Antibody (H-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-MRP-L50 Antibody (H-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MRP-L50 Antibody (H-10) Specifications

Alternate Product Name: MRP-L50

Product Clone Name: H-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-159

Epitope Target: MRP-L50

Epitope Species: mouse

Product Reactivity/Detection: MRP-L50

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for MRP-L50 Antibody (H-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin