MRG1 Antibody (JA22): sc-21795
- MRG1 Antibody (JA22) is a mouse monoclonal IgG1 κ MRG1 antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 66-270 of human MRG1, requires amino acids 66-124 for recognition
- MRG1 Antibody (JA22) is recommended for detection of MRG1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-MRG1 Antibody (JA22) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-21795 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MRG1 Antibody (JA22) for WB and IHC(P) applications. These reagents are now offered in bundles with MRG1 Antibody (JA22) (see ordering information below).
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MRG1 Antibody (JA22) is a mouse monoclonal IgG1 kappa light chain antibody that detects MRG1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MRG1 antibody (JA22) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MRG1 protein, also known as MSG1-related gene 1, plays a crucial role as a primary response gene and shares significant sequence similarity with the carboxy-terminal region of MSG1 (melanocyte-specific gene-1). Both MRG1 and MSG1 contain two conserved domains, CR1 and CR2, with CR2 being essential for transcriptional activation, indicating that they belong to a unique family of transcription factors. MRG1 is primarily located in the nucleus, where MRG1 regulates gene expression in response to various cytokines, including interleukin-1 alpha, interleukin-9, and granulocyte-macrophage colony-stimulating factor, as well as serum growth factors, through the JAK/STAT signaling pathway. Nuclear localization of MRG1 is vital for function in transcriptional regulation, as nuclear presence allows interaction with other transcription factors and co-activators, thereby influencing cellular responses to external stimuli. Notably, overexpression of MRG1 has been linked to oncogenic properties, such as anchorage-independent growth and tumor formation in nude mice, highlighting potential role in cancer biology. Additionally, a splice variant of MRG1, known as p35srj, is ubiquitously expressed and has been shown to interact with the p300-CH1 domain of p300/CBP, inhibiting interaction with hypoxia-inducible factor-1 alpha to prevent HIF-1 transactivation, further underscoring significance in cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MRG1 Antibody (JA22) References:
- Molecular cloning and chromosomal localization of the human CITED2 gene encoding p35srj/Mrg1. | Leung, MK., et al. 1999. Genomics. 61: 307-13. PMID: 10552932
- msg1, a novel melanocyte-specific gene, encodes a nuclear protein and is associated with pigmentation. | Shioda, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 12298-303. PMID: 8901575
- MSG1 and its related protein MRG1 share a transcription activating domain. | Shioda, T., et al. 1997. Gene. 204: 235-41. PMID: 9434189
- Msg1 and Mrg1, founding members of a gene family, show distinct patterns of gene expression during mouse embryogenesis. | Dunwoodie, SL., et al. 1998. Mech Dev. 72: 27-40. PMID: 9533950
- MRG1, the product of a melanocyte-specific gene related gene, is a cytokine-inducible transcription factor with transformation activity. | Sun, HB., et al. 1998. Proc Natl Acad Sci U S A. 95: 13555-60. PMID: 9811838
- Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. | Bhattacharya, S., et al. 1999. Genes Dev. 13: 64-75. PMID: 9887100
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MRG1 Antibody (JA22) | sc-21795 | 200 µg/ml | $316.00 | |||
MRG1 Antibody (JA22): m-IgG Fc BP-HRP Bundle | sc-526618 | 200 µg Ab; 10 µg BP | $354.00 | |||
MRG1 Antibody (JA22): m-IgG1 BP-HRP Bundle | sc-531991 | 200 µg Ab; 20 µg BP | $354.00 | |||
MRG1 Antibody (JA22) X | sc-21795 X | 200 µg/0.1 ml | $316.00 | |||
MRG1 Antibody (JA22) AC | sc-21795 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MRG1 Antibody (JA22) HRP | sc-21795 HRP | 200 µg/ml | $316.00 | |||
MRG1 Antibody (JA22) FITC | sc-21795 FITC | 200 µg/ml | $330.00 | |||
MRG1 Antibody (JA22) PE | sc-21795 PE | 200 µg/ml | $343.00 | |||
MRG1 Antibody (JA22) Alexa Fluor® 488 | sc-21795 AF488 | 200 µg/ml | $357.00 | |||
MRG1 Antibody (JA22) Alexa Fluor® 546 | sc-21795 AF546 | 200 µg/ml | $357.00 | |||
MRG1 Antibody (JA22) Alexa Fluor® 594 | sc-21795 AF594 | 200 µg/ml | $357.00 | |||
MRG1 Antibody (JA22) Alexa Fluor® 647 | sc-21795 AF647 | 200 µg/ml | $357.00 | |||
MRG1 Antibody (JA22) Alexa Fluor® 680 | sc-21795 AF680 | 200 µg/ml | $357.00 | |||
MRG1 Antibody (JA22) Alexa Fluor® 790 | sc-21795 AF790 | 200 µg/ml | $357.00 |