Mpi Antibody (E-4): sc-393477

Mpi Antibody (E-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Mpi in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Mpi antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mpi, or mannose phosphate isomerase, is a crucial 423 amino acid zinc metalloenzyme that plays a vital role in glycoprotein biosynthesis by catalyzing the interconversion of fructose-6-phosphate and mannose-6-phosphate, thereby ensuring a steady supply of D-mannose derivatives necessary for glycosylation reactions. This enzyme is predominantly localized in the cytoplasm and is expressed in all tissues, with higher levels found in the heart, brain, and skeletal muscle, highlighting its importance in metabolic processes across various cell types. Mutations in the gene encoding Mpi can lead to congenital disorder of glycosylation type 1B (CDG1B), a serious condition characterized by protein-losing enteropathy and often resulting in severe mental and psychomotor retardation, underscoring the critical nature of Mpi in maintaining proper cellular function and development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Mpi Antibody (E-4) References:

1. Genomic organization of the human phosphomannose isomerase (MPI) gene and mutation analysis in patients with congenital disorders of glycosylation type Ib (CDG-Ib).  |  Schollen, E., et al. 2000. Hum Mutat. 16: 247-52. PMID: 10980531
2. A broad spectrum of clinical presentations in congenital disorders of glycosylation I: a series of 26 cases.  |  de Lonlay, P., et al. 2001. J Med Genet. 38: 14-9. PMID: 11134235
3. DHPLC analysis as a platform for molecular diagnosis of congenital disorders of glycosylation (CDG).  |  Schollen, E., et al. 2002. Eur J Hum Genet. 10: 643-8. PMID: 12357336
4. Protein losing enteropathy-hepatic fibrosis syndrome in Saguenay-Lac St-Jean, Quebec is a congenital disorder of glycosylation type Ib.  |  Vuillaumier-Barrot, S., et al. 2002. J Med Genet. 39: 849-51. PMID: 12414827
5. The clinical spectrum of phosphomannose isomerase deficiency, with an evaluation of mannose treatment for CDG-Ib.  |  de Lonlay, P. and Seta, N. 2009. Biochim Biophys Acta. 1792: 841-3. PMID: 19101627
6. Exogenous mannose does not raise steady state mannose-6-phosphate pools of normal or N-glycosylation-deficient human fibroblasts.  |  Higashidani, A., et al. 2009. Mol Genet Metab. 96: 268-72. PMID: 19157945
7. In vivo and in vitro folding of a recombinant metalloenzyme, phosphomannose isomerase.  |  Proudfoot, AE., et al. 1996. Biochem J. 318 (Pt 2): 437-42. PMID: 8809030
8. Carbohydrate-deficient glycoprotein syndrome type Ib. Phosphomannose isomerase deficiency and mannose therapy.  |  Niehues, R., et al. 1998. J Clin Invest. 101: 1414-20. PMID: 9525984
9. Phosphomannose isomerase deficiency: a carbohydrate-deficient glycoprotein syndrome with hepatic-intestinal presentation.  |  Jaeken, J., et al. 1998. Am J Hum Genet. 62: 1535-9. PMID: 9585601

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mpi Antibody (E-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Mpi Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mpi Antibody (E-4) Specifications

Alternate Product Name: Mpi

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-164

Epitope Target: Mpi

Epitope Species: human

Product Reactivity/Detection: Mpi

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Mpi Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Mpi antibody is Mpi Antibody (B-2): sc-393484.