Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Morc3 CRISPR/Cas9 KO Plasmid (h): sc-404099
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Morc3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Morc3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Morc3 Antibody (G-12): sc-514672
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Morc3 CRISPR/Cas9 KO Plasmid (h) | sc-404099 | 20 µg | $397.00 |
Overview
MORC3 (Morc3) encodes a nuclear MORC family ATPase that associates with chromatin and contributes to ATP-dependent regulation of genome organization. It has been implicated in transcriptional control, maintenance of heterochromatin states, and DNA damage response processes through interactions with chromatin-associated protein complexes. MORC3 activity is linked to regulation of nuclear architecture and stress-responsive gene programs, making it relevant to studies of epigenetic stability and innate immune signaling. Dysregulated MORC3 function and autoantibody reactivity have been reported in autoimmune contexts, supporting investigation of its role in inflammation-associated cellular phenotypes.
Morc3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MORC3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MORC3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MORC3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Morc3 protein expression.
This CRISPR knockout system enables efficient generation of MORC3-deficient cell models for investigation of Morc3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MORC3 exon(s) critical for Morc3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MORC3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Morc3 CRISPR/Cas9 KO Plasmid (h) and Morc3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MORC3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Morc3 HDR Plasmid (h) and Morc3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MORC3 homology arms to support homology-directed repair at defined MORC3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.