MOBP CRISPR/Cas9 KO Plasmid (m): sc-421685
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MOBP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MOBP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MOBP Antibody (4C2): sc-517016
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MOBP CRISPR/Cas9 KO Plasmid (m) | sc-421685 | 20 µg | $397.00 |
Overview
Myelin-associated oligodendrocyte basic protein (MOBP), encoded by the mouse Mobp gene, is an abundant structural component of central nervous system myelin produced by mature oligodendrocytes. MOBP contributes to myelin compaction and stability and is commonly studied in the context of oligodendrocyte differentiation, axon–glia interactions, and maintenance of white matter integrity. Mobp expression is linked to myelin biogenesis programs and cytoskeletal and membrane organization processes that support sheath formation. Altered MOBP levels and myelin disruption are relevant to demyelinating and neurodegenerative disease mechanisms, providing a readout for white matter pathology in experimental models.
MOBP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mobp gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mobp together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mobp open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MOBP protein expression.
This CRISPR knockout system enables efficient generation of Mobp-deficient cell models for investigation of MOBP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mobp exon(s) critical for MOBP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mobp genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MOBP CRISPR/Cas9 KO Plasmid (m) and MOBP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mobp locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MOBP HDR Plasmid (m) and MOBP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mobp homology arms to support homology-directed repair at defined Mobp target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.