MMP9 Antibody (2C3): sc-21733

MMP-9 Antibody (2C3) is a mouse monoclonal IgG1 kappa light chain antibody, specifically raised against amino acids 603-614 of human MMP-9. Anti-MMP-9 antibody (2C3) demonstrates strong reactivity with human MMP-9 in a variety of applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). MMP-9, also known as gelatinase B or 92 kDa type IV collagenase, is part of the matrix metalloproteinase (MMP) family, which is critical for the breakdown of extracellular matrix proteins such as collagen, gelatin, and laminin. The structure of MMP-9 includes several key domains, such as a pro-peptide region, a catalytic domain containing zinc-binding motifs essential for enzymatic function, and a hemopexin-like domain that plays a role in substrate specificity and interaction with tissue inhibitors of metalloproteinases (TIMPs). The zinc and calcium dependence of MMP-9 is crucial for catalytic activity, as zinc ions are directly involved in the coordination and hydrolysis of substrate peptides. MMP-9 is involved in tissue remodeling processes, including bone resorption, where MMP-9 acts alongside other MMPs like MMP-1. Dysregulation of MMP-9 has been linked to pathological conditions, including joint degradation, emphasizing MMP-9′s importance in studying extracellular matrix dynamics. MMP-9 (2C3) antibody serves as a valuable tool for detecting MMP-9 in various research applications, providing insights into extracellular matrix remodeling.

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MMP9 Antibody (2C3) References:

1. p53 down-regulates human matrix metalloproteinase-1 (Collagenase-1) gene expression.  |  Sun, Y., et al. 1999. J Biol Chem. 274: 11535-40. PMID: 10206959
2. Cloning and characterization of human tumor cell interstitial collagenase.  |  Templeton, NS., et al. 1990. Cancer Res. 50: 5431-7. PMID: 2167156
3. Localization of matrix metalloproteinase 9 (92-kilodalton gelatinase/type IV collagenase = gelatinase B) in osteoclasts: implications for bone resorption.  |  Okada, Y., et al. 1995. Lab Invest. 72: 311-22. PMID: 7898050
4. High expression of 92-kD type IV collagenase (gelatinase B) in the osteoclast lineage during mouse development.  |  Reponen, P., et al. 1994. J Cell Biol. 124: 1091-1102. PMID: 8132709
5. Structural implications for the role of the N terminus in the 'superactivation' of collagenases. A crystallographic study.  |  Reinemer, P., et al. 1994. FEBS Lett. 338: 227-33. PMID: 8307185
6. Matrix metalloproteinases: a review.  |  Birkedal-Hansen, H., et al. 1993. Crit Rev Oral Biol Med. 4: 197-250. PMID: 8435466
7. Expression of several matrix metalloproteinase genes in human monocytic cells.  |  Machein, U. and Conca, W. 1997. Adv Exp Med Biol. 421: 247-51. PMID: 9330704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MMP9 Antibody (2C3) has 325 citations in a variety of scientific publications.

Primary antibodies like Anti-MMP9 Antibody (2C3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MMP9 Antibody (2C3) Specifications

Alternate Product Name: MMP-9

Product Clone Name: 2C3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 603-614

Epitope Target: MMP-9

Epitope Species: human

Product Reactivity/Detection: MMP-9

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 325 citations in various scientific publications

Available Conjugates/Forms for MMP9 Antibody (2C3): AC: Agarose, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790