Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MMP-21 CRISPR/Cas9 KO Plasmid (h): sc-414131
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MMP-21 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MMP-21 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MMP-21 Antibody (C-7): sc-398935
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MMP-21 CRISPR/Cas9 KO Plasmid (h) | sc-414131 | 20 µg | $397.00 |
Overview
MMP21 encodes matrix metalloproteinase-21 (MMP-21), a secreted zinc-dependent endopeptidase that contributes to extracellular matrix remodeling by cleaving matrix and cell-surface substrates. As part of the MMP protease network, MMP-21 influences pericellular proteolysis, basement membrane dynamics, and cell–matrix signaling that shape epithelial–mesenchymal interactions. These activities intersect with pathways linked to migration and invasion programs, including integrin-mediated adhesion, growth factor bioavailability, and inflammatory tissue remodeling. Altered MMP21 expression has been reported in multiple pathological contexts where aberrant matrix turnover accompanies disease progression, supporting its value as a mechanistic node in matrix biology.
MMP-21 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MMP21 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MMP21 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MMP21 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MMP-21 protein expression.
This CRISPR knockout system enables efficient generation of MMP21-deficient cell models for investigation of MMP-21 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MMP21 exon(s) critical for MMP-21 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MMP21 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MMP-21 CRISPR/Cas9 KO Plasmid (h) and MMP-21 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MMP21 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MMP-21 HDR Plasmid (h) and MMP-21 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MMP21 homology arms to support homology-directed repair at defined MMP21 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.