MMP2 Antibody (4D3): sc-53630
- MMP2 Antibody (4D3) is a mouse monoclonal IgG1 κ, cited in 88 publications, provided at 200 µg/ml
- raised against amino acids 557-569 of MMP-2 of human origin
- Anti-MMP2 Antibody (4D3) is recommended for detection of MMP-2 of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including rabbit; may cross-react with MMP-3
- Anti-MMP2 Antibody (4D3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for MMP2 Antibody (4D3) for WB and IHC(P) applications. This reagent is now offered in a bundle with MMP2 Antibody (4D3) (see ordering information below).
MMP-2 Antibody (4D3) is a mouse monoclonal IgG1 kappa light chain antibody, developed to recognize and bind to the amino acid residues 557-569 of the human Matrix metalloproteinase-2 (MMP-2) protein, while also demonstrating reactivity with MMP-2 from mouse, rat, and additional species including rabbit, and potential cross-reactivity with MMP-3. MMP-2 monoclonal antibody (4D3) is recommended for use in various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). MMP-2, also called gelatinase A, CLG4, MONA, CLG4A, and TBE-1, is a crucial enzyme within the matrix metalloproteinase (MMP) family, characterized by its complex structure comprising a signal peptide, a propeptide domain that maintains the enzyme in an inactive state, a catalytic domain containing a zinc-binding motif essential for its proteolytic activity, and a hemopexin-like domain that confers substrate specificity and mediates interactions with tissue inhibitors of metalloproteinases (TIMPs). The structural integrity of MMP-2 is vital for its function in degrading extracellular matrix (ECM) components such as collagen types IV, V, VII, and X, as well as gelatin type I, facilitating processes like tissue remodeling, angiogenesis, and wound healing. The propeptide domain must be cleaved to activate MMP-2, a process regulated by the Ras signaling pathway and dependent on the presence of calcium and zinc ions for catalytic activity. The hemopexin-like domain not only dictates substrate specificity but also plays a pivotal role in the enzyme′s interaction with inhibitors, thereby regulating activity within the cellular environment. Anti-MMP-2 antibody (clone 4D3) is recommended for researchers studying the role of MMP-2 in extracellular matrix remodeling, angiogenesis, and wound healing, as well as regulation and interactions within the matrix metalloproteinase family and inhibitors. Available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, MMP-2 (4D3) antibody caters to diverse experimental requirements.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MMP2 Antibody (4D3) | sc-53630 | 200 µg/ml | $316.00 | |||
MMP2 Antibody (4D3): m-IgG Fc BP-HRP Bundle | sc-525614 | 200 µg Ab; 10 µg BP | $354.00 | |||
MMP2 Antibody (4D3) AC | sc-53630 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MMP2 Antibody (4D3) HRP | sc-53630 HRP | 200 µg/ml | $316.00 | |||
MMP2 Antibody (4D3) FITC | sc-53630 FITC | 200 µg/ml | $330.00 | |||
MMP2 Antibody (4D3) PE | sc-53630 PE | 200 µg/ml | $343.00 | |||
MMP2 Antibody (4D3) Alexa Fluor® 488 | sc-53630 AF488 | 200 µg/ml | $357.00 | |||
MMP2 Antibody (4D3) Alexa Fluor® 546 | sc-53630 AF546 | 200 µg/ml | $357.00 | |||
MMP2 Antibody (4D3) Alexa Fluor® 594 | sc-53630 AF594 | 200 µg/ml | $357.00 | |||
MMP2 Antibody (4D3) Alexa Fluor® 647 | sc-53630 AF647 | 200 µg/ml | $357.00 | |||
MMP2 Antibody (4D3) Alexa Fluor® 680 | sc-53630 AF680 | 200 µg/ml | $357.00 | |||
MMP2 Antibody (4D3) Alexa Fluor® 790 | sc-53630 AF790 | 200 µg/ml | $357.00 |