MLK4 Antibody (E-11): sc-398697
- MLK4 Antibody (E-11) is a mouse monoclonal IgG1 κ MLK4 antibody provided at 200 µg/ml
- raised against amino acids 375-437 mapping within an internal region of MLK4 of human origin
- MLK4 Antibody (E-11) is recommended for detection of MLK4 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-MLK4 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MLK4 Antibody (E-11) for WB applications. These reagents are now offered in bundles with MLK4 Antibody (E-11) (see ordering information below).
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MLK4 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects MLK4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MLK4 (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MLK4 plays a crucial role in cellular signaling pathways, particularly in neuronal cells, where MLK4 is involved in mediating stress responses and apoptosis. MLK4 structure features two leucine zipper-like motifs that facilitate protein dimerization, along with a kinase domain that shares similarities with both tyrosine-specific and serine/threonine-specific kinases. This structural configuration is essential for MLK4 function, as MLK4 interacts with other signaling molecules, such as Rac1/Cdc42 and mitogen-activated protein kinase (MAPK) pathways, which are vital for regulating cell survival and death. MLK4′s ability to integrate signals from various pathways underscores MLK4′s importance in maintaining cellular homeostasis and responding to stress, making MLK4 a significant target for research in neurodegenerative diseases and cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MLK4 Antibody (E-11) References:
- The MLK family mediates c-Jun N-terminal kinase activation in neuronal apoptosis. | Xu, Z., et al. 2001. Mol Cell Biol. 21: 4713-24. PMID: 11416147
- Mixed-lineage kinase control of JNK and p38 MAPK pathways. | Gallo, KA. and Johnson, GL. 2002. Nat Rev Mol Cell Biol. 3: 663-72. PMID: 12209126
- Specific modulation of astrocyte inflammation by inhibition of mixed lineage kinases with CEP-1347. | Falsig, J., et al. 2004. J Immunol. 173: 2762-70. PMID: 15294995
- Kinase domain mutation of MLK4 gene is uncommon in gastric and hepatocellular carcinomas. | Soung, YH., et al. 2006. Dig Liver Dis. 38: 283. PMID: 16309983
- MST/MLK2, a member of the mixed lineage kinase family, directly phosphorylates and activates SEK1, an activator of c-Jun N-terminal kinase/stress-activated protein kinase. | Hirai, Si., et al. 1997. J Biol Chem. 272: 15167-73. PMID: 9182538
- The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3. | Nagata, Ki., et al. 1998. EMBO J. 17: 149-58. PMID: 9427749
- Dimerization via tandem leucine zippers is essential for the activation of the mitogen-activated protein kinase kinase kinase, MLK-3. | Leung, IW. and Lassam, N. 1998. J Biol Chem. 273: 32408-15. PMID: 9829970
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MLK4 Antibody (E-11) | sc-398697 | 200 µg/ml | $316.00 | |||
MLK4 Antibody (E-11): m-IgG Fc BP-HRP Bundle | sc-527799 | 200 µg Ab; 10 µg BP | $354.00 | |||
MLK4 Antibody (E-11): m-IgG1 BP-HRP Bundle | sc-533172 | 200 µg Ab; 20 µg BP | $354.00 | |||
MLK4 Antibody (E-11) AC | sc-398697 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MLK4 Antibody (E-11) HRP | sc-398697 HRP | 200 µg/ml | $316.00 | |||
MLK4 Antibody (E-11) FITC | sc-398697 FITC | 200 µg/ml | $330.00 | |||
MLK4 Antibody (E-11) PE | sc-398697 PE | 200 µg/ml | $343.00 | |||
MLK4 Antibody (E-11) Alexa Fluor® 488 | sc-398697 AF488 | 200 µg/ml | $357.00 | |||
MLK4 Antibody (E-11) Alexa Fluor® 546 | sc-398697 AF546 | 200 µg/ml | $357.00 | |||
MLK4 Antibody (E-11) Alexa Fluor® 594 | sc-398697 AF594 | 200 µg/ml | $357.00 | |||
MLK4 Antibody (E-11) Alexa Fluor® 647 | sc-398697 AF647 | 200 µg/ml | $357.00 | |||
MLK4 Antibody (E-11) Alexa Fluor® 680 | sc-398697 AF680 | 200 µg/ml | $357.00 | |||
MLK4 Antibody (E-11) Alexa Fluor® 790 | sc-398697 AF790 | 200 µg/ml | $357.00 |