Anti-MLK4 Antibody (E-11) is a mouse monoclonal IgG1 (kappa light chain) MLK4 antibody provided at 200 µg/ml
raised against amino acids 375-437 mapping within an internal region of MLK4 of human origin
Anti-MLK4 Antibody (E-11) is recommended for detection of MLK4 of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-MLK4 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MLK4 (E-11): sc-398697.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for MLK4 Antibody (E-11) for WB applications. These reagents are now offered in bundles with MLK4 Antibody (E-11) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
MLK4 Antibody (E-11) is a high quality monoclonal MLK4 antibody (also designated MLK4 antibody) suitable for the detection of the MLK4 protein of mouse, rat and human origin. MLK4 Antibody (E-11) is available as both the non-conjugated anti-MLK4 antibody form, as well as multiple conjugated forms of anti-MLK4 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Mixed lineage kinases are a family of protein kinases sharing two leucine zipper-like motifs, which are known to mediate protein dimerization and a kinase domain whose primary structure is similar to both the tyrosine-specific and the serine/threonine-specific kinase classes. Members of the mixed-lineage kinase (MLK) family include MLK1, MLK2, MLK3, MLK4, MELK, LZK and dual leucine zipper kinase, also designated DLK. MLKs are expressed in neuronal cells where they are likely to interact between Rac1/Cdc42, MKK4 and MKK7 in death signaling.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended fixation method for immunofluorescence staining with MLK4 (E-11): sc-398697 monoclonal antibody?
Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
Rated 5 out of
Produced excellent immunofluorescence cytoskeletalProduced excellent immunofluorescence cytoskeletal staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-24
Rated 5 out of
Good Western Blot data of MLK4 expression in UGood Western Blot data of MLK4 expression in U-251-MG, Jurkat and K-562 whole cell lysates and human liver tissue extract . -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn