Anti-MLK4 Antibody (E-11) recommended for detection of MLK4 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-MLK4 Antibody (E-11): sc-398697

Anti-MLK4 Antibody (E-11) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-MLK4 Antibody (E-11) is a mouse monoclonal IgG1 (kappa light chain) MLK4 antibody provided at 200 µg/ml
    • raised against amino acids 375-437 mapping within an internal region of MLK4 of human origin
    • Anti-MLK4 Antibody (E-11) is recommended for detection of MLK4 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-MLK4 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MLK4 (E-11): sc-398697.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for MLK4 Antibody (E-11) for WB applications. These reagents are now offered in bundles with MLK4 Antibody (E-11) (see ordering information below).

What is the recommended fixation method for immunofluorescence staining with MLK4 (E-11): sc-398697 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Produced excellent immunofluorescence cytoskeletal Produced excellent immunofluorescence cytoskeletal staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-24
Rated 5 out of 5 by from Good Western Blot data of MLK4 expression in U Good Western Blot data of MLK4 expression in U-251-MG, Jurkat and K-562 whole cell lysates and human liver tissue extract . -SCBT QC
Date published: 2013-04-22
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