MLK3 Antibody (B-12): sc-166873
- MLK3 Antibody (B-12) is a mouse monoclonal mouse IgG1 κ κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 828-847 at the C-terminus of MLK3 of human origin
- recommended for detection of MLK3 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MLK3 Antibody (B-12) for WB applications. These reagents are now offered in bundles with MLK3 Antibody (B-12) (see ordering information below).
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MLK3 Antibody (B-12) is a mouse monoclonal IgG1 antibody that detects MLK3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MLK3, also known as PTK1, is a novel human non-receptor protein kinase consisting of 847 amino acids and is notable for unique structural features, including the presence of an SH3 domain without an accompanying SH2 domain. This structural arrangement allows MLK3 to interact with proline-rich sequences in target proteins, facilitating critical signaling pathways that regulate various cellular processes. MLK3 contains a leucine zipper basic region, a motif often associated with transcription factors, suggesting a potential role in gene regulation. A long carboxy-terminal tail of MLK3, rich in proline motifs, enhances interaction with other signaling intermediates, thereby influencing cellular responses to growth factors. Given widespread expression and relationship to previously characterized MLK1 and MLK2 kinases, anti-MLK3 antibody (B-12) serves as a valuable tool for researchers studying signaling networks governing cell growth and differentiation.
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MLK3 Antibody (B-12) References:
- Induced, selective proteolysis of MLK3 negatively regulates MLK3/JNK signalling. | Liou, GY., et al. 2010. Biochem J. 427: 435-43. PMID: 20158498
- MLK3 is critical for breast cancer cell migration and promotes a malignant phenotype in mammary epithelial cells. | Chen, J., et al. 2010. Oncogene. 29: 4399-411. PMID: 20514022
- MLK3 regulates paxillin phosphorylation in chemokine-mediated breast cancer cell migration and invasion to drive metastasis. | Chen, J. and Gallo, KA. 2012. Cancer Res. 72: 4130-40. PMID: 22700880
- MLK3 promotes metabolic dysfunction induced by saturated fatty acid-enriched diet. | Gadang, V., et al. 2013. Am J Physiol Endocrinol Metab. 305: E549-56. PMID: 23860122
- MLK3 promotes melanoma proliferation and invasion and is a target of microRNA-125b. | Zhang, J., et al. 2014. Clin Exp Dermatol. 39: 376-84. PMID: 24635082
- EGFR Signals through a DOCK180-MLK3 Axis to Drive Glioblastoma Cell Invasion. | Misek, SA., et al. 2017. Mol Cancer Res. 15: 1085-1095. PMID: 28487380
- Protective role of downregulated MLK3 in myocardial adaptation to chronic hypoxia. | He, S., et al. 2016. J Physiol Biochem. 73: 371-380. PMID: 28555332
- MLK3 regulates FRA-1 and MMPs to drive invasion and transendothelial migration in triple-negative breast cancer cells. | Rattanasinchai, C., et al. 2017. Oncogenesis. 6: e345. PMID: 28604765
- Mechanisms underlying melanoma invasion as a consequence of MLK3 loss. | Balinda, HU., et al. 2022. Exp Cell Res. 415: 113106. PMID: 35307409
- MLK3 Regulates Inflammatory Response via Activation of AP-1 Pathway in HEK293 and RAW264.7 Cells. | Ha, AT., et al. 2022. Int J Mol Sci. 23: PMID: 36142785
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MLK3 Antibody (B-12) | sc-166873 | 200 µg/ml | $322.00 | |||
MLK3 Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-540171 | 200 µg Ab; 10 µg BP | $361.00 | |||
MLK3 Antibody (B-12): m-IgG1 BP-HRP Bundle | sc-541947 | 200 µg Ab; 20 µg BP | $361.00 | |||
MLK3 (B-12) Neutralizing Peptide | sc-166873 P | 100 µg/0.5 ml | $69.00 |