MLH3 Antibody (H-2): sc-25313
- MLH3 Antibody (H-2) is a mouse monoclonal IgG1 κ MLH3 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 1228-1453 of MLH3 of human origin
- MLH3 Antibody (H-2) is recommended for detection of MLH3 of mouse, rat, human origin by WB, IP, IF and ELISA
- Anti-MLH3 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MLH3 Antibody (H-2) for WB applications. These reagents are now offered in bundles with MLH3 Antibody (H-2) (see ordering information below).
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MLH3 Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects MLH3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MLH3 (H-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MLH3 protein plays a critical role in the DNA mismatch repair (MMR) pathway, which is essential for maintaining genomic stability during DNA replication. MLH3 is primarily located in the nucleus, where MLH3 partners with MLH1 to form a heterodimer that is crucial for recognizing and repairing DNA mismatches. MLH3′s nuclear localization enables direct interaction with other key proteins involved in MMR, such as MSH2 and MSH3, which are responsible for detecting mismatches. MLH3′s effective functioning in the nucleus is vital, as defects in MLH3 can lead to microsatellite instability and are associated with hereditary nonpolyposis colorectal cancer (HNPCC) and other malignancies. Using anti-MLH3 antibody (H-2) in research can provide valuable insights into DNA repair mechanisms and MMR deficiencies in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MLH3 Antibody (H-2) References:
- Eukaryotic DNA mismatch repair. | Kolodner, RD. and Marsischky, GT. 1999. Curr Opin Genet Dev. 9: 89-96. PMID: 10072354
- Different mutator phenotypes in Mlh1- versus Pms2-deficient mice. | Yao, X., et al. 1999. Proc Natl Acad Sci U S A. 96: 6850-5. PMID: 10359802
- MLH3: a DNA mismatch repair gene associated with mammalian microsatellite instability. | Lipkin, SM., et al. 2000. Nat Genet. 24: 27-35. PMID: 10615123
- Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the removal of frameshift intermediates in budding yeast. | Harfe, BD., et al. 2000. Curr Biol. 10: 145-8. PMID: 10679328
- Mismatch repair and cancer. | Palombo, F., et al. 1994. Nature. 367: 417. PMID: 8107798
- Mutation of a mutL homolog in hereditary colon cancer. | Papadopoulos, N., et al. 1994. Science. 263: 1625-9. PMID: 8128251
- Genomic organization and expression of the human MSH3 gene. | Watanabe, A., et al. 1996. Genomics. 31: 311-8. PMID: 8838312
- Tumour susceptibility and spontaneous mutation in mice deficient in Mlh1, Pms1 and Pms2 DNA mismatch repair. | Prolla, TA., et al. 1998. Nat Genet. 18: 276-9. PMID: 9500552
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MLH3 Antibody (H-2) | sc-25313 | 200 µg/ml | $322.00 | |||
MLH3 Antibody (H-2): m-IgG Fc BP-HRP Bundle | sc-526647 | 200 µg Ab; 10 µg BP | $361.00 | |||
MLH3 Antibody (H-2): m-IgG1 BP-HRP Bundle | sc-532020 | 200 µg Ab; 20 µg BP | $361.00 | |||
MLH3 Antibody (H-2) AC | sc-25313 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MLH3 Antibody (H-2) HRP | sc-25313 HRP | 200 µg/ml | $322.00 | |||
MLH3 Antibody (H-2) FITC | sc-25313 FITC | 200 µg/ml | $336.00 | |||
MLH3 Antibody (H-2) PE | sc-25313 PE | 200 µg/ml | $349.00 | |||
MLH3 Antibody (H-2) Alexa Fluor® 488 | sc-25313 AF488 | 200 µg/ml | $364.00 | |||
MLH3 Antibody (H-2) Alexa Fluor® 546 | sc-25313 AF546 | 200 µg/ml | $364.00 | |||
MLH3 Antibody (H-2) Alexa Fluor® 594 | sc-25313 AF594 | 200 µg/ml | $364.00 | |||
MLH3 Antibody (H-2) Alexa Fluor® 647 | sc-25313 AF647 | 200 µg/ml | $364.00 | |||
MLH3 Antibody (H-2) Alexa Fluor® 680 | sc-25313 AF680 | 200 µg/ml | $364.00 | |||
MLH3 Antibody (H-2) Alexa Fluor® 790 | sc-25313 AF790 | 200 µg/ml | $364.00 |