MLH1 Antibody (A-8): sc-133228
- MLH1 Antibody (A-8) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 457-756 mapping at the C-terminus of MLH1 of human origin
- recommended for detection of MLH1 of mouse, rat and human origin by WB, IP, IF and ELISA
- TransCruz reagent for ChIP application (sc-133228 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for MLH1 Antibody (A-8) for WB applications. These reagents are now offered in bundles with MLH1 Antibody (A-8) (see ordering information below).
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MLH1 Antibody (A-8) is a mouse monoclonal IgG2a antibody that detects MLH1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MLH1 plays a crucial role in the DNA mismatch repair (MMR) pathway, which is vital for maintaining genetic stability by correcting DNA replication errors. MLH1 (A-8) antibody enables detection of deficiencies that can lead to microsatellite instability and an increased risk of various cancers, including hereditary nonpolyposis colorectal cancer (HNPCC). MLH1 is predominantly located in the nucleus, where MLH1 forms a complex with other MMR proteins to identify and repair mismatched DNA bases. This localization is significant because MLH1 allows for immediate response to DNA damage, thereby preventing the accumulation of mutations that could lead to tumorigenesis. Expression of MLH1 is often lost in several cancer types, including certain ovarian tumors, which can be attributed to gene methylation or mutations. Anti-MLH1 antibody (A-8) is particularly useful for researchers studying the implications of MMR deficiencies in cancer biology and therapeutic responses, as MLH1 (A-8) monoclonal antibody provides a reliable means to assess MLH1 expression levels in various tissues and cell lines.
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MLH1 Antibody (A-8) References:
- Licensing MLH1 sites for crossover during meiosis. | Martín, AC., et al. 2014. Nat Commun. 5: 4580. PMID: 25098240
- Evaluation of MLH1 variants of unclear significance. | Köger, N., et al. 2018. Genes Chromosomes Cancer. 57: 350-358. PMID: 29520894
- HDAC6 regulates DNA damage response via deacetylating MLH1. | Zhang, M., et al. 2019. J Biol Chem. 294: 5813-5826. PMID: 30770470
- The MLH1 polymorphism rs1800734 and risk of endometrial cancer with microsatellite instability. | Russell, H., et al. 2020. Clin Epigenetics. 12: 102. PMID: 32641106
- MLH1 Deficiency Induces Cetuximab Resistance in Colon Cancer via Her-2/PI3K/AKT Signaling. | Han, Y., et al. 2020. Adv Sci (Weinh). 7: 2000112. PMID: 32670759
- Tissue-specific reduction in MLH1 expression induces microsatellite instability in intestine of Mlh1+/- mice. | Shrestha, KS., et al. 2021. DNA Repair (Amst). 106: 103178. PMID: 34311271
- MLH1 promoter hypermethylation predicts poorer prognosis in mismatch repair deficiency endometrial carcinomas. | Kaneko, E., et al. 2021. J Gynecol Oncol. 32: e79. PMID: 34431253
- Inhibition of ABL1 by tyrosine kinase inhibitors leads to a downregulation of MLH1 by Hsp70-mediated lysosomal protein degradation. | Daniels, HG., et al. 2022. Front Genet. 13: 940073. PMID: 36338985
- A novel germline frameshift mutation in the MLH1 gene in a patient with Lynch syndrome. | Xu, J., et al. 2023. Cancer Genet. 274-275: 54-58. PMID: 37001204
- Identifying primary and secondary MLH1 epimutation carriers displaying low-level constitutional MLH1 methylation using droplet digital PCR and genome-wide DNA methylation profiling of colorectal cancers. | Joo, JE., et al. 2023. Clin Epigenetics. 15: 95. PMID: 37270516
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MLH1 Antibody (A-8) | sc-133228 | 200 µg/ml | $322.00 | |||
MLH1 Antibody (A-8): m-IgG Fc BP-HRP Bundle | sc-539871 | 200 µg Ab; 10 µg BP | $361.00 | |||
MLH1 Antibody (A-8): m-IgG2a BP-HRP Bundle | sc-546804 | 200 µg Ab; 10 µg BP | $361.00 | |||
MLH1 Antibody (A-8) X | sc-133228 X | 200 µg/0.1 ml | $322.00 |