MKP-3 Antibody (G-12): sc-166041
- MKP-3 Antibody (G-12) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-130 mapping at the N-terminus of MKP-3 of human origin
- recommended for detection of MKP-3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- See MKP-3 (F-12): sc-377070 for MKP-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MKP-3 Antibody (G-12) for WB applications. These reagents are now offered in bundles with MKP-3 Antibody (G-12) (see ordering information below).
MKP-3 Antibody (G-12) is a mouse monoclonal IgG2b antibody that detects MKP-3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MKP-3, a member of the dual specificity phosphatase family, plays a crucial role in regulating mitogen-activated protein (MAP) kinase signaling pathways by specifically dephosphorylating critical phosphothreonine and phosphotyrosine residues within ERK family members. This regulation is vital for maintaining cellular homeostasis and responding to various stimuli, as dysregulation of MAP kinases can lead to pathological conditions such as cancer and inflammatory diseases. MKP-3 is encoded by a gene located on chromosome 12q22-q23 and produces a 381 amino acid protein that is predominantly expressed in the heart and pancreas, highlighting its importance in tissue-specific signaling and function. Anti-MKP-3 antibody (G-12) serves as an essential tool for researchers investigating MAP kinase-related pathways and their implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MKP-3 Antibody (G-12) References:
- Solution structure of ERK2 binding domain of MAPK phosphatase MKP-3: structural insights into MKP-3 activation by ERK2. | Farooq, A., et al. 2001. Mol Cell. 7: 387-99. PMID: 11239467
- MKP3 mediates the cellular response to FGF8 signalling in the vertebrate limb. | Kawakami, Y., et al. 2003. Nat Cell Biol. 5: 513-9. PMID: 12766772
- CD40-modulated dual-specificity phosphatases MAPK phosphatase (MKP)-1 and MKP-3 reciprocally regulate Leishmania major infection. | Srivastava, N., et al. 2011. J Immunol. 186: 5863-72. PMID: 21471446
- MEK/ERK pathway mediates insulin-promoted degradation of MKP-3 protein in liver cells. | Feng, B., et al. 2012. Mol Cell Endocrinol. 361: 116-23. PMID: 22521266
- Mapping MKP-3/FOXO1 interaction and evaluating the effect on gluconeogenesis. | Jiao, P., et al. 2012. PLoS One. 7: e41168. PMID: 22848439
- Nε-carboxymethyllysine-mediated endoplasmic reticulum stress promotes endothelial cell injury through Nox4/MKP-3 interaction. | Lee, WJ., et al. 2014. Free Radic Biol Med. 74: 294-306. PMID: 25014566
- Structural and Dynamic Insights into the Mechanism of Allosteric Signal Transmission in ERK2-Mediated MKP3 Activation. | Lu, C., et al. 2017. Biochemistry. 56: 6165-6175. PMID: 29077400
- The testosterone metabolite 3α-androstanediol inhibits oxidative stress-induced ERK phosphorylation and neurotoxicity in SH-SY5Y cells through an MKP3/DUSP6-dependent mechanism. | Mendell, AL. and MacLusky, NJ. 2019. Neurosci Lett. 696: 60-66. PMID: 30552945
- Scoparone attenuates PD-L1 expression in human breast cancer cells by MKP-3 upregulation. | Kim, SW., et al. 2024. Anim Cells Syst (Seoul). 28: 55-65. PMID: 38348341
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MKP-3 Antibody (G-12) | sc-166041 | 200 µg/ml | $322.00 | |||
MKP-3 Antibody (G-12): m-IgGκ BP-HRP Bundle | sc-534697 | 200 µg Ab; 40 µg BP | $361.00 | |||
MKP-3 Antibody (G-12): m-IgG2b BP-HRP Bundle | sc-549282 | 200 µg Ab; 10 µg BP | $361.00 |