MIZIP CRISPR/Cas9 KO Plasmid (m): sc-426433
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MIZIP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MIZIP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MIZIP Antibody (E-4): sc-398514
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MIZIP CRISPR/Cas9 KO Plasmid (m) | sc-426433 | 20 µg | $397.00 |
Overview
Zmynd19 encodes MIZIP, a MYC-interacting zinc finger–associated protein implicated in the regulation of transcriptional programs linked to cell growth and differentiation. Through its association with MYC and putative zinc finger–mediated protein complexes, MIZIP is thought to influence gene expression networks that coordinate proliferation, cellular metabolism, and lineage specification. Altered MYC-centric transcriptional control is broadly relevant to oncogenic and developmental phenotypes, making Zmynd19 a useful node for dissecting context-dependent transcriptional regulation. In mouse systems, perturbation of MIZIP can help clarify how co-regulators shape MYC-driven pathways and downstream cellular states.
MIZIP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zmynd19 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zmynd19 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zmynd19 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MIZIP protein expression.
This CRISPR knockout system enables efficient generation of Zmynd19-deficient cell models for investigation of MIZIP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zmynd19 exon(s) critical for MIZIP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zmynd19 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MIZIP CRISPR/Cas9 KO Plasmid (m) and MIZIP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zmynd19 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MIZIP HDR Plasmid (m) and MIZIP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zmynd19 homology arms to support homology-directed repair at defined Zmynd19 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.