Mitofilin Antibody (D-3): sc-390707

Mitofilin Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Mitofilin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Mitofilin antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mitofilin, also known as IMMT, HMP, PIG4, or P87/89, is a crucial 758 amino acid inner mitochondrial membrane protein that plays a significant role in maintaining mitochondrial cristae morphology, which is vital for efficient cellular respiration. Mitofilin (D-3) monoclonal antibody targets a protein involved in the formation and organization of cristae junctions and normal tubular cristae, thereby ensuring a large surface area for the assembly of cytochromes and ATP synthase proteins. This structural integrity is essential for normal mitochondrial function, as Mitofilin directly influences ATP production and overall cell survival. Given its importance in mitochondrial dynamics, Mitofilin serves as a key player in cellular energy metabolism and is particularly expressed in heart tissue, where energy demands are high.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Mitofilin Antibody (D-3) References:

1. The mitochondrial inner membrane protein mitofilin controls cristae morphology.  |  John, GB., et al. 2005. Mol Biol Cell. 16: 1543-54. PMID: 15647377
2. Mitofilin and titin as target antigens in melanoma-associated retinopathy.  |  Pföhler, C., et al. 2007. Int J Cancer. 120: 788-95. PMID: 17131336
3. The mitochondrial inner membrane protein mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11.  |  Xie, J., et al. 2007. FEBS Lett. 581: 3545-9. PMID: 17624330
4. Identification of poly-ADP-ribosylated mitochondrial proteins after traumatic brain injury.  |  Lai, Y., et al. 2008. J Neurochem. 104: 1700-11. PMID: 17996029
5. The hippocampal proteomic analysis of senescence-accelerated mouse: implications of Uchl3 and mitofilin in cognitive disorder and mitochondria dysfunction in SAMP8.  |  Wang, Q., et al. 2008. Neurochem Res. 33: 1776-82. PMID: 18307031
6. Novel intracellular functions of apolipoproteins: the ApoO protein family as constituents of the Mitofilin/MINOS complex determines cristae morphology in mitochondria.  |  Koob, S. and Reichert, AS. 2014. Biol Chem. 395: 285-96. PMID: 24391192
7. Knockdown of mitofilin inhibits autophagy and facilitates starvation-induced apoptosis in HeLa cells.  |  Shen, M., et al. 2019. Iran J Basic Med Sci. 22: 1132-1137. PMID: 31998453
8. CHCHD10-regulated OPA1-mitofilin complex mediates TDP-43-induced mitochondrial phenotypes associated with frontotemporal dementia.  |  Liu, T., et al. 2020. FASEB J. 34: 8493-8509. PMID: 32369233
9. Parkin interacts with Mitofilin to increase dopaminergic neuron death in response to Parkinson's disease-related stressors.  |  Imam Aliagan, AD., et al. 2020. Am J Transl Res. 12: 7542-7564. PMID: 33312388
10. A novel human gene that is preferentially transcribed in heart muscle.  |  Icho, T., et al. 1994. Gene. 144: 301-6. PMID: 8039717
11. Molecular characterization of mitofilin (HMP), a mitochondria-associated protein with predicted coiled coil and intermembrane space targeting domains.  |  Odgren, PR., et al. 1996. J Cell Sci. 109 (Pt 9): 2253-64. PMID: 8886976
12. Mitofilin is a transmembrane protein of the inner mitochondrial membrane expressed as two isoforms.  |  Gieffers, C., et al. 1997. Exp Cell Res. 232: 395-9. PMID: 9168817

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mitofilin Antibody (D-3) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Mitofilin Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mitofilin Antibody (D-3) Specifications

Alternate Product Name: Mitofilin

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 364-657

Epitope Target: Mitofilin

Epitope Species: human

Product Reactivity/Detection: Mitofilin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Mitofilin Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin