MITF Antibody (4H205): sc-71587
- MITF Antibody (4H205) is a mouse monoclonal IgG1, cited in 1 publications, provided at 100 µg/ml
- raised against amino acids 408-419 MITF of human origin
- recommended for detection of MITF of mouse, rat, human, avian and Xenopus origin by WB and IP; also reactive with additional species, including and equine, bovine and canine
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MITF Antibody (4H205). This work is in progress.
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SEE ALSO...
MITF Antibody (4H205) is a mouse monoclonal IgG1 antibody that detects MITF (microphthalmia-associated transcription factor) in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. MITF is a crucial melanocytic nuclear protein that plays a significant role in the development and differentiation of melanocytes, which are responsible for pigment production in the skin, hair, and eyes. MITF features essential structural motifs, including basic helix-loop-helix (HLH) and leucine zipper (LZ) domains, which are characteristic of many transcription factors, particularly those in the Myc family. These motifs enable MITF to function as a homodimer that directly binds to DNA, activating various gene promoters by interacting with their E-boxes. MITF regulates the expression of key pigment synthesis genes such as tyrosinase, TRP1, and TRP2, which are vital for melanin production. The phosphorylation of MITF on Ser73, mediated by the MAPK pathway, enhances transcriptional activity by facilitating association with co-activators from the p300/CBP family. MITF has several isoforms, with MITF-M being specifically expressed in melanocytes; absence of MITF in mice leads to a complete lack of melanocytes, underscoring MITF′s critical role in melanocyte biology.
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MITF Antibody (4H205) References:
- A germline oncogenic MITF mutation and tumor susceptibility. | Paillerets, BB., et al. 2014. Eur J Cell Biol. 93: 71-5. PMID: 24290354
- The MITF family of transcription factors: Role in endolysosomal biogenesis, Wnt signaling, and oncogenesis. | Ploper, D. and De Robertis, EM. 2015. Pharmacol Res. 99: 36-43. PMID: 26003288
- MITF-M regulates melanogenesis in mouse melanocytes. | Chen, T., et al. 2018. J Dermatol Sci. 90: 253-262. PMID: 29496358
- MITF and UV responses in skin: From pigmentation to addiction. | Nguyen, NT. and Fisher, DE. 2019. Pigment Cell Melanoma Res. 32: 224-236. PMID: 30019545
- The transcription factor MITF in RPE function and dysfunction. | Ma, X., et al. 2019. Prog Retin Eye Res. 73: 100766. PMID: 31242455
- Endolysosomal Cation Channels and MITF in Melanocytes and Melanoma. | Abrahamian, C. and Grimm, C. 2021. Biomolecules. 11: PMID: 34356645
- MiTF/TFE Translocation Renal Cell Carcinomas: From Clinical Entities to Molecular Insights. | Simonaggio, A., et al. 2022. Int J Mol Sci. 23: PMID: 35886994
- Joint contribution of MC1R and MITF alleles to melanoma risk in the age of polygenic risk scores. | Bruno, W. 2023. Br J Dermatol. 188: 696-697. PMID: 36975215
- MRGPRX2 signaling involves the Lysyl-tRNA synthetase and MITF pathway. | Guo, Y., et al. 2023. Front Immunol. 14: 1154108. PMID: 37234172
- Emerging roles of MITF as a crucial regulator of immunity. | Lee, A., et al. 2024. Exp Mol Med. 56: 311-318. PMID: 38351314
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MITF Antibody (4H205) | sc-71587 | 100 µg/ml | $322.00 |