MIS Antibody (B-11): sc-166752

MIS Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Müllerian inhibiting substance (MIS) protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MIS antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MIS, a member of the transforming growth factor beta (TGFβ) superfamily, plays a crucial role in sexual differentiation during embryonic development by inhibiting the development of the Müllerian ducts in male embryos, thereby preventing the formation of female reproductive structures. This function ensures proper development of male reproductive organs, and absence can lead to disorders of sexual development. Additionally, MIS has been implicated in tumor suppression, particularly in tumors derived from Müllerian duct tissues, highlighting potential as a therapeutic target in certain cancers. MIS production occurs in Sertoli cells of the testes, and secretion, along with testosterone, is essential for male offspring development. The presence of both hormones leads to regression of the Müllerian ducts and differentiation of the Wolffian ducts, establishing male reproductive anatomy.

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MIS Antibody (B-11) References:

1. Müllerian inhibiting substance in reproduction and cancer.  |  Donahoe, PK. 1992. Mol Reprod Dev. 32: 168-72. PMID: 1637555
2. Mullerian Inhibiting Substance is an ovarian growth factor of emerging clinical significance.  |  Seifer, DB. and Maclaughlin, DT. 2007. Fertil Steril. 88: 539-46. PMID: 17559842
3. The role of mullerian inhibiting substance in female reproduction.  |  Nakhuda, GS. 2008. Curr Opin Obstet Gynecol. 20: 257-64. PMID: 18460940
4. Utility of serum antimüllerian hormone/Müllerian-inhibiting substance for predicting ovarian reserve in older women.  |  Berin, I. and Teixeira, J. 2008. Menopause. 15: 824-6. PMID: 18600185
5. The transforming growth factor-beta family.  |  Massagué, J. 1990. Annu Rev Cell Biol. 6: 597-641. PMID: 2177343
6. Multiple type-beta transforming growth factors and their receptors.  |  Massagué, J., et al. 1987. J Cell Physiol Suppl. Suppl 5: 43-7. PMID: 2890647
7. Müllerian inhibiting substance/anti-Müllerian hormone as a fertility preservation agent.  |  Pépin, D., et al. 2018. Curr Opin Endocrinol Diabetes Obes. 25: 399-405. PMID: 30320617
8. Isolation of the bovine and human genes for Müllerian inhibiting substance and expression of the human gene in animal cells.  |  Cate, RL., et al. 1986. Cell. 45: 685-98. PMID: 3754790
9. The in vivo roles of müllerian-inhibiting substance.  |  Behringer, RR. 1994. Curr Top Dev Biol. 29: 171-87. PMID: 7828438
10. Molecular genetics of the persistent müllerian duct syndrome: a study of 19 families.  |  Imbeaud, S., et al. 1994. Hum Mol Genet. 3: 125-31. PMID: 8162013
11. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member.  |  McPherron, AC., et al. 1997. Nature. 387: 83-90. PMID: 9139826

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MIS Antibody (B-11) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-MIS Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MIS Antibody (B-11) Specifications

Alternate Product Name: Mμllerian inhibiting substance

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 541-560

Epitope Target: MIS

Epitope Species: human

Product Reactivity/Detection: precursor and mature MIS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for MIS Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin