Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MiRP1 CRISPR/Cas9 KO Plasmid (h): sc-418462
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MiRP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MiRP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MiRP1 Antibody (H-4): sc-374667
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MiRP1 CRISPR/Cas9 KO Plasmid (h) | sc-418462 | 20 µg | $397.00 |
Overview
KCNE2 encodes the human MinK-related peptide 1 (MiRP1), a single-pass transmembrane β-subunit that modulates voltage-gated potassium channel function, including KCNH2/hERG, to tune channel gating, trafficking, and membrane repolarization. Through its effects on cardiac action potential duration and epithelial ion transport, MiRP1 contributes to electrical excitability and electrolyte homeostasis in tissues such as heart and gastrointestinal epithelium. Perturbation of KCNE2-dependent channel regulation is linked to altered repolarization reserve and arrhythmia susceptibility, and has also been studied in relation to thyroid and gastric physiology. As a modulatory subunit, MiRP1 provides a mechanistic entry point for dissecting ion channel macromolecular complexes, membrane protein quality control, and stimulus-dependent changes in excitability.
MiRP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCNE2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCNE2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCNE2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MiRP1 protein expression.
This CRISPR knockout system enables efficient generation of KCNE2-deficient cell models for investigation of MiRP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCNE2 exon(s) critical for MiRP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCNE2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MiRP1 CRISPR/Cas9 KO Plasmid (h) and MiRP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCNE2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MiRP1 HDR Plasmid (h) and MiRP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCNE2 homology arms to support homology-directed repair at defined KCNE2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.