MICA/B Antibody (F-6): sc-137242

MICA/B Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects MICA/B of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MICA/B (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MICA and MICB proteins are stress-induced antigens closely related to major histocompatibility complex (MHC) class I molecules, and are frequently expressed in epithelial tumors. These highly glycosylated cell surface proteins are unique in that MICA/B proteins are stably expressed without conventional class I peptide ligands or association with β-2-Microglobulin, which is crucial for function in immune response. MICA and MICB proteins are primarily located on epithelial cell surfaces, where expression is induced during cellular stress, such as proliferation or heat shock. Surface localization allows direct interaction with immune cells, particularly intestinal epithelial Vδ1 γδ T cells, which recognize MICA/B through variable T cell receptors. This interaction suggests MICA and MICB play a vital role in signaling cellular distress, thereby evoking immune responses in intestinal epithelium, making MICA/B proteins important targets for research in cancer immunology and therapeutic interventions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MICA/B Antibody (F-6) References:

1. Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB.  |  Groh, V., et al. 1999. Proc Natl Acad Sci U S A. 96: 6879-84. PMID: 10359807
2. MICA rather than MICB, TNFA, or HLA-DRB1 is associated with susceptibility to psoriatic arthritis.  |  González, S., et al. 2002. J Rheumatol. 29: 973-8. PMID: 12022360
3. Antibody-mediated inhibition of MICA and MICB shedding promotes NK cell-driven tumor immunity.  |  Ferrari de Andrade, L., et al. 2018. Science. 359: 1537-1542. PMID: 29599246
4. Cocultures of human colorectal tumor spheroids with immune cells reveal the therapeutic potential of MICA/B and NKG2A targeting for cancer treatment.  |  Courau, T., et al. 2019. J Immunother Cancer. 7: 74. PMID: 30871626
5. MICa/b-dependent activation of natural killer cells by CD64+ inflammatory type 2 dendritic cells contributes to autoimmunity.  |  Sánchez-Cerrillo, I., et al. 2023. EMBO J. 42: e113714. PMID: 37916875
6. Inhibition of MICA and MICB shedding enhances memory-like NK-cell-mediated cytotoxicity against multiple myeloma.  |  Tahri, S., et al. 2024. Blood Adv. 8: 5365-5370. PMID: 39106432
7. A second lineage of mammalian major histocompatibility complex class I genes.  |  Bahram, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 6259-63. PMID: 8022771
8. Nucleotide sequence of the human MHC class I MICA gene.  |  Bahram, S., et al. 1996. Immunogenetics. 44: 80-1. PMID: 8613147
9. Cell stress-regulated human major histocompatibility complex class I gene expressed in gastrointestinal epithelium.  |  Groh, V., et al. 1996. Proc Natl Acad Sci U S A. 93: 12445-50. PMID: 8901601
10. Genomic structure of the human MHC class I MICB gene.  |  Bahram, S., et al. 1996. Immunogenetics. 45: 161-2. PMID: 8952966
11. Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells.  |  Groh, V., et al. 1998. Science. 279: 1737-40. PMID: 9497295
12. Diversification, expression, and gamma delta T cell recognition of evolutionarily distant members of the MIC family of major histocompatibility complex class I-related molecules.  |  Steinle, A., et al. 1998. Proc Natl Acad Sci U S A. 95: 12510-5. PMID: 9770516

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MICA/B Antibody (F-6) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-MICA/B Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MICA/B Antibody (F-6) Specifications

Alternate Product Name: MHC Class I Related Chain

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: MICA

Epitope Species: human

Product Reactivity/Detection: MICA and MICB

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for MICA/B Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin