MICA Antibody (2C10): sc-23870
- MICA Antibody (2C10) is a mouse monoclonal IgG1 κ MICA antibody, cited in 22 publications, provided at 200 µg/ml
- raised against C1R cells expressing MICA mRNA after stable transfection with MICA cDNA in RSV.5neo
- MICA Antibody (2C10) is recommended for detection of MICA of human origin by WB, IP and FCM
- Anti-MICA Antibody (2C10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MICA Antibody (2C10) for WB applications. These reagents are now offered in bundles with MICA Antibody (2C10) (see ordering information below).
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MICA Antibody (2C10) is a mouse monoclonal IgG1 kappa light chain antibody that detects the MICA protein of human origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). MICA (2C10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MICA, a member of the major histocompatibility complex (MHC) class I family, plays a crucial role in the immune response by acting as a stress-induced ligand for natural killer (NK) cells and certain T cells. MICA expression is particularly important in tumor immunology, as MICA is often upregulated in epithelial tumors, signaling cellular distress and potentially triggering immune responses. This highly glycosylated cell surface protein is unique in expressing independently of conventional class I peptide ligands and not requiring association with beta-2-microglobulin. MICA recognition by intestinal epithelial Vδ1 γδ T cells, which express diverse T cell receptors, underscores MICA′s significance in mediating immune surveillance and responses in the intestinal epithelium, particularly during conditions of cellular stress.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MICA Antibody (2C10) References:
- Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB. | Groh, V., et al. 1999. Proc Natl Acad Sci U S A. 96: 6879-84. PMID: 10359807
- MICA in kidney transplants. | Allison, SJ. 2022. Nat Rev Nephrol. 18: 273. PMID: 35338343
- MICA is a histocompatibility antigen in transplantation. | Li, XC. 2022. Am J Transplant. 22: 2129. PMID: 36039544
- MICA and MICB allele assortment in Finland. | Koskela, S., et al. 2023. HLA. 102: 52-61. PMID: 36919857
- MICA-specific nanobodies for diagnosis and immunotherapy of MICA+ tumors. | Verhaar, ER., et al. 2024. Front Immunol. 15: 1368586. PMID: 38550583
- Nanobody-based CAR NK cells for possible immunotherapy of MICA+ tumors. | Verhaar, ER., et al. 2024. PNAS Nexus. 3: pgae184. PMID: 38756234
- A second lineage of mammalian major histocompatibility complex class I genes. | Bahram, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 6259-63. PMID: 8022771
- Allelic repertoire of the human MHC class I MICA gene. | Fodil, N., et al. 1996. Immunogenetics. 44: 351-7. PMID: 8781120
- Cell stress-regulated human major histocompatibility complex class I gene expressed in gastrointestinal epithelium. | Groh, V., et al. 1996. Proc Natl Acad Sci U S A. 93: 12445-50. PMID: 8901601
- Genomic structure of the human MHC class I MICB gene. | Bahram, S., et al. 1996. Immunogenetics. 45: 161-2. PMID: 8952966
- Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. | Groh, V., et al. 1998. Science. 279: 1737-40. PMID: 9497295
- Diversification, expression, and gamma delta T cell recognition of evolutionarily distant members of the MIC family of major histocompatibility complex class I-related molecules. | Steinle, A., et al. 1998. Proc Natl Acad Sci U S A. 95: 12510-5. PMID: 9770516
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MICA Antibody (2C10) | sc-23870 | 200 µg/ml | $316.00 | |||
MICA Antibody (2C10): m-IgG Fc BP-HRP Bundle | sc-528374 | 200 µg Ab; 10 µg BP | $354.00 | |||
MICA Antibody (2C10): m-IgGκ BP-HRP Bundle | sc-520736 | 200 µg Ab, 40 µg BP | $354.00 | |||
MICA Antibody (2C10): m-IgG1 BP-HRP Bundle | sc-542901 | 200 µg Ab; 20 µg BP | $354.00 | |||
MICA Antibody (2C10) AC | sc-23870 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MICA Antibody (2C10) HRP | sc-23870 HRP | 200 µg/ml | $316.00 | |||
MICA Antibody (2C10) FITC | sc-23870 FITC | 200 µg/ml | $330.00 | |||
MICA Antibody (2C10) PE | sc-23870 PE | 200 µg/ml | $343.00 | |||
MICA Antibody (2C10) Alexa Fluor® 488 | sc-23870 AF488 | 200 µg/ml | $357.00 | |||
MICA Antibody (2C10) Alexa Fluor® 546 | sc-23870 AF546 | 200 µg/ml | $357.00 | |||
MICA Antibody (2C10) Alexa Fluor® 594 | sc-23870 AF594 | 200 µg/ml | $357.00 | |||
MICA Antibody (2C10) Alexa Fluor® 647 | sc-23870 AF647 | 200 µg/ml | $357.00 | |||
MICA Antibody (2C10) Alexa Fluor® 680 | sc-23870 AF680 | 200 µg/ml | $357.00 | |||
MICA Antibody (2C10) Alexa Fluor® 790 | sc-23870 AF790 | 200 µg/ml | $357.00 |