mHMGCS Antibody (B-8): sc-393256

mHMGCS Antibody (B-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects mHMGCS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-mHMGCS antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. mHMGCS, or mitochondrial HMG-CoA synthase, plays a crucial role in ketone body biosynthesis by catalyzing the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is subsequently converted into mevalonate by HMG-CoA reductase, initiating the isoprenoid biosynthesis pathway. This pathway is vital for producing essential biomolecules, including cholesterol and ubiquinone, which are critical for cellular membrane integrity and energy production, respectively. mHMGCS is predominantly expressed in liver and kidney, and expression is regulated by various metabolic states; fasting, cyclic adenosine monophosphate, and fatty acids enhance transcription, while feeding and insulin exert a repressive effect. Additionally, a regulatory element within mHMGCS promoter allows for transcriptional regulation by nuclear receptors such as PPAR, RXR, COUP-TF, and HNF-4, highlighting mHMGCS′s importance in metabolic regulation and energy homeostasis.

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mHMGCS Antibody (B-8) References:

1. Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase: a control enzyme in ketogenesis.  |  Hegardt, FG. 1999. Biochem J. 338 (Pt 3): 569-82. PMID: 10051425
2. Sterol regulatory element binding protein-mediated effect of fluvastatin on cytosolic 3-hydroxy-3-methylglutaryl-coenzyme A synthase transcription.  |  Mascaró, C., et al. 2000. Arch Biochem Biophys. 374: 286-92. PMID: 10666309
3. Sterol regulatory element-binding proteins (SREBPs) as regulators of lipid metabolism: polyunsaturated fatty acids oppose cholesterol-mediated induction of SREBP-1 maturation.  |  Kim, HJ., et al. 2002. Ann N Y Acad Sci. 967: 34-42. PMID: 12079833
4. Mitochondrial HMG-CoA synthase deficiency: identification of two further patients carrying two novel mutations.  |  Wolf, NI., et al. 2003. Eur J Pediatr. 162: 279-80. PMID: 12647205
5. Amplification and direct sequencing of a cDNA encoding human cytosolic 3-hydroxy-3-methylglutaryl-coenzyme A synthase.  |  Russ, AP., et al. 1992. Biochim Biophys Acta. 1132: 329-31. PMID: 1358203
6. Refining the diagnosis of mitochondrial HMG-CoA synthase deficiency.  |  Aledo, R., et al. 2006. J Inherit Metab Dis. 29: 207-11. PMID: 16601895
7. Rat mitochondrial and cytosolic 3-hydroxy-3-methylglutaryl-CoA synthases are encoded by two different genes.  |  Ayté, J., et al. 1990. Proc Natl Acad Sci U S A. 87: 3874-8. PMID: 1971108
8. Human mitochondrial HMG CoA synthase: liver cDNA and partial genomic cloning, chromosome mapping to 1p12-p13, and possible role in vertebrate evolution.  |  Boukaftane, Y., et al. 1994. Genomics. 23: 552-9. PMID: 7851882
9. Molecular cloning and tissue expression of human mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase.  |  Mascaró, C., et al. 1995. Arch Biochem Biophys. 317: 385-90. PMID: 7893153
10. The role of mitochondrial HMG-CoA synthase in regulation of ketogenesis.  |  Quant, PA. 1994. Essays Biochem. 28: 13-25. PMID: 7925316
11. The hepatocyte nuclear factor 4 (HNF-4) represses the mitochondrial HMG-CoA synthase gene.  |  Rodríguez, JC., et al. 1998. Biochem Biophys Res Commun. 242: 692-6. PMID: 9464279
12. Transcriptional regulation of mitochondrial HMG-CoA synthase in the control of ketogenesis.  |  Hegardt, FG. 1998. Biochimie. 80: 803-6. PMID: 9893938

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-mHMGCS Antibody (B-8) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-mHMGCS Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

mHMGCS Antibody (B-8) Specifications

Alternate Product Name: mHMGCS

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 419-488

Epitope Target: mHMGCS

Epitope Species: human

Product Reactivity/Detection: mHMGCS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for mHMGCS Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin