MHC class I Antibody (ER-MP42) detects MHC class I molecules on the surface of cells of the following haplotypes: H2-Fv, H2-Dd, H2k,q, s and, to a lesser extent, H2p,r,w7,w22 of mouse origin by IF and FCM

MHC class I Antibody (ER-MP42): sc-59200

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Datasheets

    • MHC class I Antibody (ER-MP42) is a rat monoclonal IgG2a provided at 200 µg/ml
    • raised against macrophage precursor cells of mouse origin
    • recommended for detection of MHC class I molecules on the surface of cells of the following haplotypes: H2-Fv, H2-Dd, H2k,q, s and, to a lesser extent, H2p,r,w7,w22 of mouse origin by IF and FCM; non cross-reactive with MHC class I molecules of other haplotypes
    • See MHC class I (ER-HR52): sc-59199 for MHC class I antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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If I want to use MHC class I (ER-MP42): sc-59200 for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: DefinitelyNotMatt
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-24
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