MGMT Antibody (MT 3.1): sc-56157
- MGMT Antibody (MT 3.1) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against MGMT of human origin
- MGMT Antibody (MT 3.1) is recommended for detection of MGMT of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and FCM
- Anti-MGMT Antibody (MT 3.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for MGMT Antibody (MT 3.1) for WB and IHC(P) applications. This reagent is now offered in a bundle with MGMT Antibody (MT 3.1) (see ordering information below).
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MGMT Antibody (MT 3.1) is a mouse monoclonal IgG1 kappa light chain antibody raised against MGMT of human origin, showing reactivity with MGMT from human and, to a lesser extent, mouse and rat samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). MGMT, also known as O6-methylguanine-DNA methyltransferase or O-6-alkylguanine DNA alkyltransferase (AGT), is a crucial DNA repair enzyme predominantly located in the cell nucleus. Nuclear localization of MGMT allows direct access to damaged DNA, where anti-MGMT antibody (MT 3.1) removes mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs, thus maintaining genomic stability. MGMT activity is essential for protecting cells from DNA alkylation damage, and MGMT expression levels can influence the effectiveness of certain chemotherapeutic agents. High MGMT expression is often associated with resistance to alkylating drugs. MGMT expression is transcriptionally activated in response to DNA damage, and this induction requires functional p53, a tumor suppressor protein involved in DNA repair and apoptosis. Studies have shown that high MGMT activity correlates with mutant p53 in some cancer cell lines, suggesting a complex regulatory relationship. Understanding cellular localization and regulation of MGMT is important for cancer research and therapeutic development, making MGMT monoclonal antibody (MT 3.1) a valuable tool for researchers investigating DNA repair mechanisms and p53-related pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MGMT Antibody (MT 3.1) References:
- Activity of O(6)-methylguanine-DNA methyltransferase in relation to p53 status and therapeutic response in ovarian cancer. | Hengstler, JG., et al. 1999. Int J Cancer. 84: 388-95. PMID: 10404091
- High O6-methylguanine methyl transferase activity is frequently found in human oral cancer cells with p53 inactivation. | Guo, W., et al. 1999. Int J Oncol. 15: 817-21. PMID: 10493967
- Mammalian O6-alkylguanine-DNA alkyltransferase: regulation and importance in response to alkylating carcinogenic and therapeutic agents. | Pegg, AE. 1990. Cancer Res. 50: 6119-29. PMID: 2205376
- Importance of the DNA repair enzyme O6-alkyl guanine alkyltransferase (AT) in cancer chemotherapy. | D'Incalci, M., et al. 1988. Cancer Treat Rev. 15: 279-92. PMID: 3071420
- Contribution of O6-alkylguanine and N-alkylpurines to the formation of sister chromatid exchanges, chromosomal aberrations, and gene mutations: new insights gained from studies of genetically engineered mammalian cell lines. | Kaina, B., et al. 1993. Environ Mol Mutagen. 22: 283-92. PMID: 8223512
- Induction of murine O6-alkylguanine-DNA-alkyltransferase in response to ionising radiation is p53 gene dose dependent. | Rafferty, JA., et al. 1996. Oncogene. 12: 693-7. PMID: 8637727
- p53 is involved in regulation of the DNA repair gene O6-methylguanine-DNA methyltransferase (MGMT) by DNA damaging agents. | Grombacher, T., et al. 1998. Oncogene. 17: 845-51. PMID: 9780001
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MGMT Antibody (MT 3.1) | sc-56157 | 200 µg/ml | $322.00 | |||
MGMT Antibody (MT 3.1): m-IgG Fc BP-HRP Bundle | sc-525650 | 200 µg Ab; 10 µg BP | $361.00 | |||
MGMT Antibody (MT 3.1) AC | sc-56157 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MGMT Antibody (MT 3.1) HRP | sc-56157 HRP | 200 µg/ml | $322.00 | |||
MGMT Antibody (MT 3.1) FITC | sc-56157 FITC | 200 µg/ml | $336.00 | |||
MGMT Antibody (MT 3.1) PE | sc-56157 PE | 200 µg/ml | $349.00 | |||
MGMT Antibody (MT 3.1) Alexa Fluor® 488 | sc-56157 AF488 | 200 µg/ml | $364.00 | |||
MGMT Antibody (MT 3.1) Alexa Fluor® 546 | sc-56157 AF546 | 200 µg/ml | $364.00 | |||
MGMT Antibody (MT 3.1) Alexa Fluor® 594 | sc-56157 AF594 | 200 µg/ml | $364.00 | |||
MGMT Antibody (MT 3.1) Alexa Fluor® 647 | sc-56157 AF647 | 200 µg/ml | $364.00 | |||
MGMT Antibody (MT 3.1) Alexa Fluor® 680 | sc-56157 AF680 | 200 µg/ml | $364.00 | |||
MGMT Antibody (MT 3.1) Alexa Fluor® 790 | sc-56157 AF790 | 200 µg/ml | $364.00 |